Mouse cytokine and acute phase protein Taqman primer and probe sequences
| Target | Accession number | Oligonucleotide | Sequence | Amplicon size (bp) |
|---|---|---|---|---|
| SAA | M13522 | Forward primer | 5′-GCCATGGAGGGTTTTTTTCATT-3′ | 80 |
| Reverse primer | 5′-CCTTTGGGCAGCATCATAGTTC-3′ | |||
| Probe | 5′-CACATGTCTCCAGCCCCTTGGAAAGC-3′ | |||
| α2-AP | Y12312 | Forward primer | 5′GCGGTTCACAGTGTCGGT-3′ | 66 |
| Reverse primer | 5′-TCCAGCAGGAACCATCGAA-3′ | |||
| Probe | 5′-ACATGATGCACGCGGTGTCATATCCT-3′ | |||
| C3 | J00367 | Forward primer | 5′-CCATGTATTCCATCATTACTCCCAA-3′ | 72 |
| Reverse primer | 5′-CGTGGGCCTCCAGTCAGA-3′ | |||
| Probe | 5′-CCTACGGCTGGAGAGCGAAGAGACC-3′ | |||
| PTX3 | X83601 | Forward primer | 5′-ACAACGAAATAGACAATGGACTTCAT-3′ | 62 |
| Reverse primer | 5′-CTGGCGGCAGTCGCA-3′ | |||
| Probe | 5′-CCACCGAGGACCCCACGCC-3′ | |||
| IL-1β | M15131 | Forward primer | 5′-GCCACCCACCCTGCA-3′ | 69 |
| Reverse primer | 5′-ACCGCTTTTCCATCTTCTTCTT-3′ | |||
| Probe | 5′-TGGAGAGTCTGGATCCCAAGCAATACCC-3′ | |||
| TNFα | M11731 | Forward primer | 5′-CTCCAGGCGGTGCCTATG-3′ | 149 |
| Reverse primer | 5′-GGGCCATAGAACTGATGAGAGG-3′ | |||
| Probe | 5′-TCAGCCTCTTCTCATTCCTGCTTGTGG-3′ | |||
| IL-6 | NM_031168 | Forward primer | 5′-TCCAGAAACCGCTATGAAGTTC-3′ | 72 |
| Reverse primer | 5′-CACCAGCATCAGTCCCAAGA-3′ | |||
| Probe | 5′-CTCTGCAAGAGACTTCCATCCAGTTGCC-3′ | |||
| TGFβ1 | AJ009862 | Forward primer | 5′-CGTGGAAATCAACGGGATCA-3′ | 84 |
| Reverse primer | 5′-GGCCATGAGGAGCAGGAA-3′ | |||
| Probe | 5′-ACCTGGGCACCATCCATGACATGA-3′ | |||
| iN0S | NM_010927 | Forward primer | 5′-CAGCTGGGCTGTACAAACCTT-3′ | 95 |
| Reverse primer | 5′-CATTGGAAGTGAAGCGTTTCG-3′ | |||
| Probe | 5′-CGGGCAGCCTGTGAGACCTTTGA-3′ |