Table 2.

The effect of 3 μm etomidate on the properties of mIPSCs and the tonic current of VB neurons of WT, β2N265S, and β2–/– mice

Control +3 μm Etomidate
VB neurons WT β2N265S β2-/- WT β2N265S β2-/-
mIPSC amplitude (pA) 69 ± 5 94 ± 8 20 ± 2 73 ± 7 79 ± 10 22 ± 1.9
mIPSC τW (ms) 5 ± 0.8 3.5 ± 0.1 ND 14.4 ± 2.3* 5.9 ± 0.4* ND
mIPSC charge (fC) 357 ± 30 435 ± 70 66 ± 11 834 ± 101* 531 ± 42* 82 ± 6
Phasic charge (fC/s) 18,779 22,881 1373 43,869 27,931 1706
Tonic current (pA) 62 ± 12 70 ± 13 28 ± 2 +152 ± 15 +60 ± 7 +42 ± 6
Tonic charge (fC/s) 62,000 70,000 28,000 +152,000 +60,000 +42,000
Total charge (fC/s) 80,779 92,881 29,373 +177,090 +65,050 +42,333
  • The effect of etomidate on the tonic current is expressed as the change in the holding current (i.e., the current induced) by the anesthetic. Total inhibitory charge induced by etomidate is calculated as the sum of the etomidate-induced change in the phasic charge and the etomidate-induced increase in the tonic current.*p < 0.01 versus control (paired Student's t test; n = 4-6 neurons). ND, Not determined