Channel | Activation | Inactivation | Recovery from inactivation | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
τ1 | τ2 | τ3 | n | ||||||||||
V1/2 (mV) | z (e0) | n | V1/2 (mV) | a (mV) | n | ms | % | ms | % | ms | % | ||
WT | −13.7 ± 0.9 | 5.3 ± 0.2 | 5 | −37.9 ± 0.9 | 6.9 ± 0.4 | 5 | 6.1 ± 2.8 | 12 ± 4 | 79 ± 32 | 46 ± 15 | 388 ± 149 | 41 ± 19 | 5 |
R859C | −5.4 ± 0.9* | 4.7 ± 0.2* | 5 | −39.7 ± 1.4* | 6.9 ± 0.4 | 5 | 6.9 ± 2.4 | 19 ± 9 | 128 ± 38 | 41 ± 13 | 613 ± 151* | 38 ± 7 | 5 |
WT plus β1 | −19.6 ± 1.9 | 6.0 ± 0.9 | 5 | −41.9 ± 1.7 | 6.6 ± 0.4 | 5 | 2.6 ± 0.3 | 94 ± 5 | 104 ± 15 | 24 ± 5 | NDa | NDa | 5 |
R859C plus β1 | −10.8 ± 1.5** | 4.4 + 0.4** | 5 | −42.4 ± 1.4 | 7.2 ± 0.5 | 5 | 2.5 ± 0.3 | 96 ± 4 | 99 ± 10 | 23 ± 5 | NDa | NDa | 5 |
WT plus R859C+β1 | −21.2 ± 3.1 | 5.1 ± 0.3** | 6 | NDb | NDb | NDb | NDb | NDb | NDb | NDb | NDb |
↵aNot determined because the recovery of these channels was best fit with a double exponential.
↵bNot determined because the inactivation and recovery from fast inactivation were not affected by the R859C mutation.
The asterisk indicates a statistically significant difference from wild-type (WT) α alone at p < 0.05; double asterisks indicate a statistically significant difference from WT α plus β1 at p < 0.05.