Cell marker | Proliferating in B104 CM | Differentiated in T3/1% FCS | Differentiated in 10% FCS |
---|---|---|---|
PSA-NCAM | + | −/+ | − |
PDGFrα | + | −/+ | − |
A2B5 | + | − | −/+ |
O4 | + | + | − |
O1 | −/+ | + | − |
MBP | − | + | − |
PLP | − | + | − |
GFAP | − | − | + |
Tuj1 | − | − | − |
Progenitors were incubated with B104 conditioned medium (CM) to promote their proliferation or in low (1%) or high (10%) FCS to stimulate their differentiation. Low serum culture medium contained T3 hormone. Cells were then immunostained with cell-lineage-specific antibodies to study the acquired phenotype in the different incubation conditions. Positive and negative indicates presence or absence of immunolabeling, respectively. Results were replicated in three independent experiments. Tuj1, Class III β-tubulin.