Table 1.

Primers used for PCR

PrimerSequence (5′–3′)aSize of the fragmentbGenBank accession number
VGluT1-FGGATCCATTTAGGTGACACTATAGAAGagatcagcaaggtgggactg894 bp U07609
VGluT1-RGAATTCTAATACGACTCACTATAGGGAGAagaaggagagagggctggtc
VGluT2-FGGATCCATTTAGGTGACACTATAGAAGtcaatgaaatccaacgtcca886 bp NM_053427
VGluT2-RGAATTCTAATACGACTCACTATAGGGAGAcaagagcacaggacaccaaa
GAD67-FGGATCCATTTAGGTGACACTATAGAAGttatgtcaatgcaaccgc812 bp NM_017007
GAD67-RGAATTCTAATACGACTCACTATAGGGAGAcccaacctctctatttcctc
PPT-FGGATCCATTTAGGTGACACTATAGAAGtccgacagtgaccaaatcaa783 bp NM_012666
PPT-RGAATTCTAATACGACTCACTATAGGGAGAcacaacacaggaaacatgctg

  • F, Forward; R, reverse.

  • ↵aCapital letters are attached sequences. T7 (reverse primer) or SP6 (forward primer) promoter sequences are underlined.

  • ↵bThe size of the fragment includes 56 bp attached sequences.