Table 1.

Averaged characteristics of FRAP curves for SEP–GluR2 and for memGFP in nonspiny and spiny (mushroom and stubby) membranes

Mobile fraction (%)t1/2 (s)Diffusion coefficient (μm2/s)n
SEP–GluR2
Flat nonspiny83.6  ± 4.111.4  ± 2.80.1329  ± 0.02816
Mushroom53.5  ± 3.9222.7  ± 25.30.0067  ± 0.001622
Stubby46.9  ± 3.8100.7  ± 29.50.0162  ± 0.00708
memGFP
Flat nonspiny86.5  ± 3.42.6  ± 0.60.6350  ± 0.07018
Mushroom92.0  ± 2.27.7  ± 1.50.1558  ± 0.029629
Stubby85.2  ± 2.75.3  ± 1.00.1746  ± 0.022612
  • Data show means ± SEM for mobile fraction, half-time of recovery, and estimated diffusion coefficient (calculated as described in Materials and Methods).