mAb | EC50 (nm) |
---|---|
7F5 | 0.26 |
11E1 | 0.05 |
17D11 | 0.07 |
19E12 | 0.05 |
29D7 | 0.47 |
SH-SY5Y cells were plated in 96-well plates at a density of 4 × 103 cells/well and incubated with 10 μ m RA for 3 d to induce neuronal differentiation. Subsequently, cells were treated with varying concentrations of indicated mAbs, and the neurite outgrowth was measured using the Cellomics ArrayScan.