Functional analysis of mutants made to region surrounding Y521 in GluR6
| Mutant | Glutamate | Kainate | ||||||
|---|---|---|---|---|---|---|---|---|
| Ipeak (nA) | τdes (ms) | %des | n | Ipeak (nA) | τdes (ms) | %des | n | |
| Wild type | 6.4 ± 0.3 | 5.7 ± 0.1 | 99.7 ± 0.04 | 80 | 4.6 ± 0.3 | 4.6 ± 0.1 | 99.5 ± 0.1 | 47 |
| K525E | 3.0 ± 0.4 | 6.3 ± 0.4 | 100 | 10 | 1.1 ± 0.2 | 3.7 ± 0.5 | 99.9 ± 0.1 | 8 |
| I780A | 0.2 ± 0.1 | 1.0 ± 0.1 | 100 | 9 | 0.03 ± 0.01 | 0.9 ± 0.2 | 100 | 4 |
| I780F | 1.4 ± 0.2 | 1.3 ± 0.1 | 100 | 6 | 0.2 ± 0.04 | 1.3 ± 0.2 | 100 | 6 |
| I780L | 9.7 ± 0.6 | 17.8 ± 1.4 | 98.1 ± 0.6 | 12 | 8.0 ± 0.7 | 42.7 ± 3.0 | 87.1 ± 1.7 | 10 |
| I780M | 6.1 ± 0.5 | 4.3 ± 0.2 | 99.9 ± 0.1 | 12 | 4.5 ± 0.5 | 4.3 ± 0.3 | 99.5 ± 0.3 | 11 |
| I780S | 2.3 ± 0.5 | 1.2 ± 0.1 | 100 | 9 | 0.5 ± 0.1 | 1.2 ± 0.1 | 100 | 9 |
| I780V | 3.7 ± 0.6 | 2.6 ± 0.1 | 100 | 10 | 2.2 ± 0.5 | 2.5 ± 0.3 | 100 | 9 |
| Q784K | 7.4 ± 0.7 | 7.6 ± 0.6 | 99.7 ± 0.2 | 12 | 6.2 ± 0.5 | 5.3 ± 0.6 | 99.6 ± 0.2 | 10 |
| K525E | 4.7 ± 0.9 | 15.1 ± 0.1 | 99.9 ± 0.03 | 12 | 3.7 ± 0.6 | 5.7 ± 0.3 | 99.7 ± 0.3 | 11 |
| Q784K | ||||||||
| Y521L | 7.4 ± 0.9 | 6.5 ± 0.4 | 99.9 ± 0.02 | 11 | 5.5 ± 0.8 | 4.9 ± 0.4 | 99.4 ± 0.2 | 10 |
| K525E | ||||||||
| Q784K | ||||||||
| K525E | 7.2 ± 0.8 | 61.5 ± 4.1 | 98.2 ± 0.7 | 10 | 6.1 ± 0.9 | 132 ± 12 | 86.9 ± 2.8 | 10 |
| I780L | ||||||||
| Q784K | ||||||||
Ipeak, τdes, and %des are peak currents, desensitization time constant, and percentage desensitization, respectively, determined as described in Materials and Methods. Values are shown mean ± SEM for n determinations. When values for Ipeak and τdes differed more than fourfold from wild type, this is indicated in bold, and it is indicated in italics when values are lower. Percentage desensitization of <95% is also highlighted in bold. The mutant K525E/I780L/Q784K is referred to as the triple mutant in the text.