Mutant | Kainate | Glutamate | ||
---|---|---|---|---|
Kd (nm) | n | Ki (nm) | n | |
Wild type | 8.9 ± 1.5 | 3 | 320 ± 29 | 3 |
K525E | 7.6 ± 2.0 | 4 | 266 ± 50 | 3 |
I780A | 10.6 ± 1.0 | 3 | 387 ± 81 | 4 |
I780L | 8.9 ± 1.3 | 3 | 337 ± 50 | 3 |
E787A | 8.3 ± 0.7 | 4 | 357 ± 41 | 3 |
E787G | 9.0 ± 0.8 | 5 | 265 ± 37 | 3 |
K525E/K696R/ | 7.4 ± 2.6 | 3 | 214 ± 7 | 3 |
I780L/Q784K |
Kd is the affinity constant determined for [3H]kainate, and Ki is the estimated affinity constant, both shown as mean ± SEM for n values, determined as described in Materials and Methods.