Table 3.

Top 50 downregulated genes during OL differentiation

Probe set ID	Fold change	Peak level	Gene name	Gene symbol
M93669_at	−49.52	1827	*Secretogranin II*	*SCG2*
J03627_at	−37.01	1855	*S100 calcium binding protein A10*	*S100A10*
rc_AA899590_at	−27.47	4001	*ESTs, no homologies found*
rc_AA899854_at	−25.81	3481	*Topoisomerase (DNA) 2α*	*TOP2A*
rc_AA997800_at	−22.01	2285	Antigen Ki-67 (predicted)	Ki-67
rc_AI228113_s_at	−19.03	591	Neuronal pentraxin receptor	NPTXR
rc_AI176963_at	−18.13	840	*Cbp/p300-interacting transactivator 2*	*CITED2*
J03624_at	−18.00	2821	*Galanin*	*GALN*
rc_AA963443_at	−18.00	996	Cyclin D2	CCND2
J02585_at	−17.27	5693	Stearoyl-coenzyme A desaturase 1	SCD1
rc_AA956638_at	−16.45	525	Ubiquitin-like, PHD + RING finger domains 1	UHRF1
rc_AA944180_at	−15.89	1429	CDC28 protein kinase regulatory subunit 2	CKS2
rc_AA818744_at	−15.56	516	Complement comp. 1, q subcomp., receptor 1	CD93
rc_AI101009_at	−14.22	1223	*Zinc finger, CCHC domain containing 12*	*ZCCHC12*
rc_AI010123_at	−14.12	1384	Transforming, acidic coiled-coil cont. protein 2	TACC2
X59864mRNA_g_at	−13.83	3102	*H19 fetal liver mRNA*	*H19*
rc_AI231702_at	−13.74	341	Centrosomal protein 152 kDa	CEP152
rc_AA819103_at	−13.45	685	Mesoderm specific transcript homolog	MEST
rc_AA997385_at	−13.36	887	Ubiquitin specific protease 43	USP43
rc_AI179576_s_at	−13.27	3573	*Hemoglobin, β*	*HBB*
X60767mRNA_s_at	−13.09	1163	Cell division cycle control protein 2	CDC2
rc_AI071227_at	−13.00	418	DNA repair protein RAD51 homolog 1	RAD51
rc_AI639088_s_at	−12.73	792	ESTs, no homologies found
rc_AA859341_at	−12.38	475	SH3 and PX domains 2A	SH3PXD2A
U17565_g_at	−12.04	3466	*Mini chromosome maintenance deficient 6*	*MCM6*
rc_AI058975_at	−11.96	1063	SRY-box containing gene 11	SOX11
rc_AI146172_at	−11.88	1565	Neuron specific gene family member 2	NSG2
rc_AA900746_at	−11.88	485	ESTs, no homologies found
M74223_at	−11.79	5120	*VGF nerve growth factor inducible*	*VGF*
rc_AI103150_at	−11.71	934	Ubiquitin-conjugating enzyme E2C	UBE2C
rc_AI010612_at	−11.47	254	Tenascin C	TNC
rc_AA956688_at	−11.39	2048	Cell division cycle associated 3	CDCA3
rc_AI231497_at	−11.31	1171	Karyopherinα2	KPNA2
rc_AA893717_at	−11.24	2174	Rac GTPase-activating protein 1	RACGAP1
rc_AI012221_at	−11.16	1413	Chloride intracellular channel 1	CLIC1
rc_AI169327_g_at	−11.08	957	Tissue inhibitor of metalloproteinase 1	TIMP1
rc_AI045594_at	−10.93	1752	Spindle pole body component 25	SPBC25
rc_AI103106_at	−10.78	1783	Lamin B1	LMNB1
rc_AI072698_at	−10.70	869	TRAF4 associated factor 1	TRAF4AF1
rc_AA851392_at	−10.63	537	Kinesin family member 22	KIF22
rc_AA818016_at	−10.56	2069	Cell division cycle 23	CDC23
rc_AI237713_at	−10.56	1007	ESTs, no homologies found
rc_AI103682_at	−10.56	307	Polymerase (DNA directed),α1	POLA
rc_AI232326_at	−10.48	989	ESTs, no homologies found
rc_AI145123_at	−10.48	989	Similar to ataxin 2-binding protein 1 isoform 2
rc_AA925012_at	−10.48	1711	Geminin	GMNN
rc_AI104821_at	−10.34	460	Leucine rich repeat interacting protein 1	LRRFIP1
AF019974_at	−10.27	381	Chromogranin B	CHGB
rc_AA943886_at	−10.20	830	Polo-like kinase 2	PLK2
rc_AI230215_at	−10.06	1336	Cell division cycle associated 8	CDCA8

The 50 unique genes most strongly repressed during OL differentiation are listed. Note that genes with a likely 2A expression pattern are not included. Genes listed in bold are present in both Tables 3 and 4. In cases in which more than one Affymetrix probe set (Probe set ID) corresponding to the same gene was highly regulated, only the most strongly regulated is listed. Fold change, Highest expression level/lowest expression level in time course; negative numbers are downregulated relative to OPCs. Peak level, Highest raw level of expression seen at any time point in the gene chip experiment.