DMSO concentration | Average number of puncta | FM4-64 uptake (a.u.) | Relative release (%) | Kinetics (s) |
---|---|---|---|---|
Untreated | 34 | 59.59 ± 3.94 | 47.57 ± 2.83 | 21.94 ± 1.03 |
0.1% | 49 | 62.73 ± 7.79 | 40.09 ± 0.83 | 25.12 ± 1.49 |
1% | 36 | 59.68 ± 6.39 | 47.8 ± 2.70 | 26.96 ± 2.33 |
2% | 32 | 65.94 ± 5.71 | 50.80 ± 5.18 | 25.88 ± 1.35 |
Cultures were left untreated or treated with DMSO at concentrations indicated for 2 h. Proteasome inhibitors clasto-lactacystin, β-lactone, and epoxomicin were dissolved in DMSO at 1% for all given experiments. Data are means ± SEM; n = 5 for all conditions.