State-dependent digestion of artificial N termini
| Construct | 0 Ca2+, 0 mV | 10 μm Ca2+, 0 mV | 10 μm Ca2+, −80 mV | |||
|---|---|---|---|---|---|---|
| τd (s) | n | τd | n | τd | n | |
| FIW4QKK24Q | 53.3 ± 7.4 | 2.6 ± 0.5 | 409.8 ± 67.8 | 1.1 ± 0.2 | 42.0 ± 7.7 | 3.0 ± 0.9 |
| FIW6QKK22Q | 59.0 ± 12.9 | 1.9 ± 0.4 | 583.0 + 44.6 | 1.0* | ||
| FIW8QKK20Q | 39.8 ± 11.3 | 1.7 ± 0.6 | 570.3 ± 206 | 0.9 ± 0.1 | ||
| FIW10QKK18Q | 50.8 ± 9.9 | 1.8 ± 0.5 | 348.8 ± 77.4 | 1.2 ± 0.1 | 25.4 ± 5.9 | 2.5 ± 0.9 |
| FIW11QKK15Q | 17.7 ± 2.7 | 3.3 ± 0.9 | 17.4 ± 1.7 | 3.7 ± 0.5 | 15.0 ± 0.6 | 4.0* |
| 325.7 ± 37.5 | 1.0* | |||||
| FIW17QKK10Q | 29.8 + 1.7 | 2.6 ± 0.22 | 48.5 ± 6.5 | 1.9 ± 0.3 | ||
| FIW20QKK8Q | 79.7 ± 8.9 | 1.7 ± 0.2 | 117.3 ± 14.9 | 1.04 ± 0.1 | 72.7 ± 8.9 | 1.7 ± 0.2 |
| FIW22QKK6Q | 74.6 ± 11.0 | 2.0* | ||||