n | Depth (μm) | Evoked responses (Hz) | ||||
---|---|---|---|---|---|---|
Aβ | Aδ | C | PD | |||
Sham rats | ||||||
Intraplantar | ||||||
Vehicle | 4 | 815 ± 51 | 203 ± 19 | 268 ± 56 | 622 ± 100 | 635 ± 188 |
URB (25 μg) | 6 | 912 ± 28 | 178 ± 24 | 179 ± 38 | 418 ± 74 | 349 ± 99 |
AM251 plus URB (25 μg) | 6 | 698 ± 64 | 143 ± 18 | 162 ± 34 | 514 ± 71 | 637 ± 106 |
Nal plus URB (25 μg) | 6 | 692 ± 61 | 156 ± 16 | 177 ± 18 | 431 ± 58 | 428 ± 83 |
Spinal | ||||||
URB (10, 25, and 50 μg) | 6 | 820 ± 40 | 163 ± 29 | 176 ± 29 | 585 ± 140 | 363 ± 120 |
AM251 plus URB (50 μg) | 6 | 744 ± 70 | 113 ± 10 | 145 ± 40 | 522 ± 82 | 492 ± 130 |
Nal plus URB (50 μg) | 5 | 730 ± 56 | 136 ± 9 | 149 ± 10 | 547 ± 46 | 519 ± 152 |
SNL rats | ||||||
Intraplantar | ||||||
Vehicle | 4 | 760 ± 138 | 167 ± 14 | 167 ± 8 | 365 ± 68 | 245 ± 50 |
URB (25 μg) | 6 | 723 ± 87 | 142 ± 21 | 151 ± 20 | 372 ± 57 | 333 ± 72 |
URB (100 μg) | 6 | 755 ± 54 | 150 ± 12 | 219 ± 29 | 580 ± 73 | 722 ± 112 |
AM251 plus URB (100 μg) | 6 | 712 ± 54 | 159 ± 9 | 184 ± 25 | 439 ± 68 | 434 ± 124 |
Nal plus URB (100 μg) | 6 | 718 ± 64 | 153 ± 12 | 148 ± 13 | 327 ± 27 | 307 ± 75 |
URB (100 μg) contralateral paw | 6 | 682 ± 48 | 147 ± 7 | 105 ± 16 | 274 ± 35 | 292 ± 36 |
Spinal | ||||||
Vehicle | 5 | 610 ± 46 | 152 ± 11 | 177 ± 34 | 439 ± 119 | 385 ± 135 |
URB (10, 25, 50 μg) | 8 | 666 ± 38 | 88 ± 8.4 | 132 ± 24 | 484 ± 77 | 554 ± 99 |
AM251 plus URB (50 μg) | 5 | 854 ± 168 | 102 ± 14 | 147 ± 20 | 598 ± 99 | 647 ± 49 |
Nal plus URB (50 μg) | 6 | 852 ± 73 | 102 ± 20 | 160 ± 13 | 417 ± 30 | 509 ± 84 |
Depths from the surface of the spinal cord and electrically (Aβ, Aβ-fiber; Aδ, Aδ-fiber; C, C-fiber; PD, postdischarge) evoked responses of neurons were statistically compared using one-way ANOVA with their respective controls. Data are expressed as mean ± SEM. n, Number of neurons per group; URB, URB597; Nal, naloxone.