GABA-positive cell numerical density in the E15 presumptive medial prefrontal cortex
| Experimental group | Mean ± SEM | t value* | p value |
|---|---|---|---|
| Entire cerebral wall (all laminas) | |||
| WT | 86.9 ± 5.9 | ||
| D1-KO | 64.8 ± 2.1 | 3.51 | 0.013 |
| D2-KO | 123.5 ± 1.9 | 5.87 | 0.001 |
| Marginal zone | |||
| WT | 34.6 ± 2.4 | ||
| D1-KO | 31.4 ± 0.8 | 1.26 | 0.25 (NS) |
| D2-KO | 37.4 ± 2.4 | 0.86 | 0.43 (NS) |
| Cortical plate + subplate | |||
| WT | 22.8 ± 2.4 | ||
| D1-KO | 18.2 ± 3.0 | 1.18 | 0.28 (NS) |
| D2-KO | 33.9 ± 6.8 | 1.62 | 0.16 (NS) |
| Intermediate zone | |||
| WT | 26.5 ± 3.0 | ||
| D1-KO | 13.8 ± 1.3 | 3.83 | 0.009 |
| D2-KO | 51.9 ± 3.3 | 5.68 | 0.001 |
| Ventricular zone + subventricular zone | |||
| WT | 3.0 ± 0.6 | ||
| D1-KO | 1.4 ± 0.3 | 2.44 | 0.02 |
| D2-KO | 7.3 ± 0.9 | 4.04 | 0.007 |
We calculated GABA-positive cell numbers in the entire cerebral wall as well as in each lamina between wild-type (WT), D1 receptor knock-out (D1-KO), and D2 receptor knock-out (D2-KO) embryos and compared the data between groups using a t test. GABA-positive cell numerical density in the E15 presumptive medial prefrontal cortex.
↵*, All comparisons are to the wild-type group; n = 4 embryos for each experimental group. NS, Not statistically significant, i.e., p > 0.05.