Antioxidant properties of metalloporphyrins
| Agent code or chemical | SOD activity (U/mg) | CAT (1/min) | 4-HNE IC50(nm) |
|---|---|---|---|
| Native Cu–Zn SOD | 15,267 | – | – |
| AEOL10150 | 43,103 | 2.2 | 271 |
| AEOL11207 | 124 | 1.54 | 71 |
SOD activity, Unit of SOD activity is defined as the amount of compound that inhibits one-half the formation of adrenochrome by superoxide at pH 10.2. CAT, Catalase activity is measured by a Clarke-type oxygen electrode (Trova et al., 2003). 4-HNE IC50, The concentration (nanomolars) of compound required to inhibit 50% 4-HNE formation in rat brain homogenates treated with FeCl2and ascorbate. For details, see Materials and Methods.