Table 1.

The CNS myelin proteome

Protein name	Accession	Acronym	IEF spot number	16BAC spot number	IEF-T	IEF-V	LC-V	LC-R	Allen
Known myelin proteins
Claudin-11, OSP	Q60771	CLD11	nd	82	nd	nd	Yes	nd	+
CNP	P16330	CN37	61, 82, 84–88, 97–100, 102–106	18,34,41–43, 45,46	nd	nd	Yes	Yes	+
Contactin 1	P12960	CNTN1	nd	7	Yes	nd	Yes	Yes	+
Myelin basic protein	P04370	MBP	182–185, 187, 200, 202, 205–207	61,62,65–73	nd	Yes	Yes	Yes	+
Myelin proteolipid protein	P60202	MYPR	nd	51,63,78,84	nd	nd	Yes	Yes	+
Myelin associated glycoprotein	P20917	MAG	nd	13	nd	nd	Yes	Yes	+
Myelin oligodendrocytic basic protein	NP032640	(NCBI)	nd	79	nd	nd	Yes	nd	+
Myelin oligodendrocyte glycoprotein	Q61885	MOG	nd	6.58	nd	nd	Yes	Yes	+
Neurofascin	Q810U3	NFASC	nd	5	nd	nd	nd	Yes	+
Transmembrane proteins
Calnexin	P35564	CALX	nd	81	nd	nd	nd	Yes	+
Contactin-associated protein 1, Caspr	O54991	CNTP1	nd	3, 4	nd	nd	nd	nd	+/−
Immunoglobulin superfamily member 8	Q8R366	IGSF8	nd	19	nd	nd	Yes	Yes	+
Na⁺K⁺-ATPase α 1	Q8VDN2	AT1A1	nd	12.15	nd	nd	Yes	Yes	+/−
Na⁺K⁺-ATPase α 2	Q6PIE5	AT1A2	nd	11	nd	nd	nd	Yes	+
Na⁺K⁺-ATPase α 3	Q6PIC6	AT1A3	nd	11.14	nd	nd	nd	Yes	+
Na⁺K⁺-ATPase β 1	P14094	AT1B1	nd	44	nd	nd	Yes	Yes	+/−
N-CAM 120	P13594	NCA12	1	nd	Yes	nd	nd	Yes	+
Thy 1 membrane glycoprotein	P01831	THY1	150	nd	nd	nd	Yes	Yes	+/−
Cytoplasmic proteins
14–3-3 protein gamma, YWHAG	P61982	1433G	147	nd	Yes	Yes	nd	Yes	+/−
Actin β	P60710	ACTB	192.193	nd	nd	Yes	Yes	Yes	+
Actin γ	P63260	ACTG	nd	48	nd	nd	nd	nd	+
ADP ribosylation factor 6, ARF6	P62331	ARF6	166	nd	nd	nd	nd	nd	+
Aldolase A, Fructose-bisphosphate	P05064	ALDOA	108	nd	Yes	Yes	Yes	Yes	nd
Argininosuccinate synthase	P16460	ASSY	nd	40	nd	nd	nd	nd	nd
Cannabinoid receptor interacting	Q5M8N0	CB032	173	nd	nd	nd	nd	nd	nd
Carbonic anhydrase 2	P00920	CAH2	145	nd	Yes	nd	Yes	nd	+
Cell division control protein 42, cdc42	P60766	CDC42	155	nd	nd	nd	nd	nd	+
Actin related protein α, Centractin	P61164	ACTZ	92	nd	nd	nd	nd	nd	+
Clathrin heavy chain	Q68FD5	CLH	nd	6	nd	nd	nd	Yes	nd
Creatine kinase brain	Q04447	KCRB	83	nd	Yes	Yes	Yes	Yes	+
Crystallin α2	P23927	CRYAB	160	nd	Yes	nd	Yes	nd	+
Dihydropyrimidinase related 2, CRMP-2	O08553	DPYSL2	15–17, 33, 36, 47, 110	23	Yes	Yes	Yes	Yes	+
Dynamin 1	P39053	DYN1	4, 5, 6, 7	12	Yes	nd	nd	Yes	+
EH-domain containing protein 1	Q9WVK4	EHD1	nd	24	Yes	nd	Yes	nd	+
EH-domain-containing protein 3	Q9QXY6	EHD3	nd	24	nd	nd	nd	nd	+
Elongation factor 1-α 1	P10126	EF1A1	194	38	nd	nd	Yes	Yes	+
Elongation factor 1-α 2	P62631	EF1A2	58	32	nd	nd	nd	nd	nd
Enolase 1	P17182	ENOA	76, 78–80, 112, 114, 118, 119	33	Yes	Yes	Yes	nd	+
Enolase 2, neuron-specific, NSE	P17183	ENOG	64	nd	Yes	Yes	Yes	nd	+
Ezrin	P26040	EZRI	18	nd	Yes	nd	nd	nd	+
Fascin	Q61553	FSCN1	74.75	nd	nd	nd	nd	nd	+
Glial fibrillary acidic protein	P03995	GFAP	65	33	nd	nd	nd	nd	+
Glucose-6-phosphate isomerase	P06745	G6PI	nd	64	nd	nd	nd	Yes	nd
Glutamine synthetase	P15105	GLNA	94–96	nd	Yes	Yes	Yes	nd	+
Glutathione S-transferase Mu 1	P10649	GSTM1	217	nd	nd	nd	nd	nd	nd
GAPDH	P16858	G3P	128.131	nd	Yes	Yes	Yes	nd	+
Guanine nucleotide-binding α2	P18873	GNAO2	nd	50	Yes	nd	nd	nd	nd
Guanine nucleotide-binding β1	P62874	GBB1	196	nd	Yes	Yes	Yes	nd	+
Guanine nucleotide-binding β2	P62880	GBB2	138.139	nd	nd	Yes	nd	Yes	+
Guanine nucleotide-binding β4	P29387	GBB4	140	nd	nd	nd	nd	nd	+
Guanine nucleotide-binding β5	P62881	GBB5	116	nd	nd	nd	nd	nd	+
Hsp 90-α, HSP89	P07901	HS90A	nd	16	nd	nd	nd	nd	+
Hspa2, Hsp70.2, Hsp72	P17156	HSP72	28, 29	20	nd	nd	nd	nd	+
Hspa5, Hsp70, Grp78, BiP	P20029	GRP78	26	nd	Yes	nd	nd	nd	+
Hsp70/Hsp90 organizing, STIP1	Q60864	STIP1	38	nd	Yes	nd	nd	nd	+
Hspa8, Hsp73	P63017	HSP7C	29	20	nd	Yes	Yes	Yes	+
Neurofilament 66 kDa, internexin α	P46660	AINX	41–43, 45, 46	25–27	Yes	Yes	nd	Yes	nd
Lactate dehydrogenase B	P16125	LDHB	211	nd	Yes	nd	nd	nd	nd
Malate dehydrogenase, cytoplasmic	P14152	MDHC	141	nd	Yes	Yes	Yes	nd	+/−
Moesin	P26041	MOES	22.198	nd	nd	nd	nd	nd	+
Munc 18, syntaxin-binding protein 1	O08599	STXB1	nd	77	Yes	nd	nd	Yes	+
Myosin 1d	Q5SYD0	MYO1D	nd	10	nd	nd	nd	Yes
Myosin 14	Q6URW6	MYH14	nd	1	nd	nd	nd	nd	nd
NDRG1, N-myc downstream regulated	Q62433	NDRG1	89	nd	Yes	Yes	Yes	nd	+
Neurofilament triplet H	P19246	NFH	197	2	nd	nd	nd	nd	+/−
Neurofilament triplet L	P08551	NFL	27.6	22	Yes	Yes	nd	Yes	+/−
Neurofilament triplet M	P08553	NFM	nd	8.9	nd	nd	nd	Yes	nd
NSF, N-ethylmaleimide sensitive fusion	P46460	NSF	21.23	75	Yes	nd	nd	Yes	+
Nucleoside diphosphate kinase A	P15532	NDKA	168.169	nd	Yes	nd	Yes	nd	nd
Nucleoside diphosphate kinase B	Q01768	NDKB	170.171	nd	Yes	nd	Yes	nd	nd
Pacsin 1	Q61644	PACN1	59	nd	nd	nd	nd	nd	+/−
Peptidyl-prolyl cis-trans isomerase A	P17742	PPIA	172	nd	nd	Yes	Yes	nd	+
Peroxiredoxin 1	P35700	PRDX1	159.165	nd	Yes	Yes	nd	Yes	+
Peroxiredoxin 2	Q61171	PRDX2	199	nd	nd	Yes	nd	nd	+/−
Phosphatidylethanolamine binding	P70296	PEBP	156	nd	nd	Yes	nd	nd	+
Phosphatidylinositol transfer α	P53810	PIPNA	142	nd	nd	nd	nd	nd	+/−
Phosphoglycerate dehydrogenase	Q61753	SERA	209	nd	nd	nd	nd	nd	nd
Phosphoglycerate mutase 1	Q9DBJ1	PGAM1	213	nd	Yes	nd	Yes	nd	+/−
PLCb1, phospholipase Cb1	Q9Z1B3	PLCB1	66	nd	Yes	nd	nd	nd	+/−
Programmed cell death 6-interacting	Q9WU78	PDC6I	4	nd	nd	nd	nd	nd	+
Protein disulfide isomerase Grp58	P27773	PDIA3	214	nd	Yes	nd	nd	nd	+
Pyruvate kinase isozyme M2	P52480	KPYM	53.54	nd	Yes	Yes	Yes	nd	+
Rab GDP dissociation inhibitor α	P50396	GDIA	48.148	nd	Yes	nd	Yes	Yes	+
Rab GDP dissociation inhibitor β	Q61598	GDIB	77	nd	Yes	nd	nd	nd	+
Radixin	P26043	RADI	19.2	nd	nd	nd	nd	nd	+
Ras-like protein Ral-A	P63321	RALA	nd	59	nd	nd	nd	nd	+
Ras-related protein Rap-1A	P62835	RAP1A	154	nd	Yes	nd	Yes	Yes	+
Septin 2	P42208	SEPT2	90, 91, DIGE	39	Yes	nd	Yes	nd	+
Septin 4	P28661	SEPT4	DIGE	nd	nd	nd	nd	nd	+
Septin 7	O55131	SEPT7	194	37	Yes	nd	Yes	Yes	+
Septin 8	Q8CHH9	SEPT8	69, 70, 71, DIGE	83	nd	Yes	Yes	Yes	+
Sirtuin 2, NAD-dependent	Q8VDQ8	SIRT2	120–127, DIGE	nd	Yes	Yes	Yes	Yes	+
Soluble NSF attachment protein α	Q9DB05	SNAA	135	nd	nd	nd	nd	nd	+
Soluble NSF attachment protein β	P28663	SNAB	136	nd	nd	nd	nd	nd	+/−
Soluble NSF attachment protein γ	Q9CWZ7	SNAG	111	nd	nd	nd	nd	nd	+/−
Spectrin α 2	P16546	SPTA2	nd	85	Yes	nd	nd	nd	+/−
Superoxide dismutase 1, Cu/Zn	P08228	SODC	168	nd	nd	nd	Yes	nd	+/−
Synapsin 1	O88935	SYN1	24.25	nd	nd	nd	nd	nd	+/−
Synapsin 2	Q64332	SYN2	215	nd	nd	nd	nd	nd	+
Synaptosomal-associated protein 25	P60879	SNP25	150	nd	nd	Yes	Yes	Yes	nd
T-complex 1 α B	P11983	TCPA2	14	nd	nd	nd	nd	nd	nd
T-complex 1 β	P80314	TCPB	73	nd	nd	nd	nd	nd	nd
T-complex 1 ϵ	P80316	TCPE	13	nd	nd	nd	nd	nd	nd
T-complex 1 γ	P80318	TCPG	37	nd	nd	nd	nd	nd	nd
Transgelin 3	Q9R1Q8	TAGL3	158	nd	nd	nd	nd	nd	+
Transitional ER ATPase	Q01853	TERA	2	nd	Yes	nd	nd	nd	nd
Transketolase	P40142	TKT	39.4	21	Yes	nd	Yes	nd	+
Tubulin α 1	P68369	TBA1	190	3.28	nd	nd	nd	Yes	+
Tubulin α 2	P05213	TBA2	189	nd	Yes	Yes	Yes	nd	nd
Tubulin β 2C	P68372	TBB2C	191	35.36	Yes	nd	Yes	Yes	nd
Tubulin β 4	Q9D6F9	TBB4	137.188	31	nd	Yes	Yes	Yes	+
Tubulin polymerization-promoting	Q7TQD2	P25A	164	nd	nd	Yes	nd	nd	+
Ubiquitin	P62991	UBIQ	181	nd	nd	nd	nd	nd	nd
Ubiquitin hydrolase L1	Q9R0P9	UCHL1	149	nd	Yes	nd	nd	nd	+/−
Vacuolar ATP synthase A, ubiquitous	P50516	VATA1	31	nd	nd	nd	nd	nd	nd
Vacuolar ATP synthase B, brain	P62814	VATB2	49.5	nd	nd	nd	nd	nd	nd
WD-repeat protein 1	O88342	WDR1	216	nd	nd	nd	nd	nd	+
Extracellular proteins
Hemoglobin α	P01942	HBA	163, 176, 177, 180, 186, 204	nd	nd	nd	Yes	Yes	nd
Hemoglobin β	P02088	HBB1	174, 175, 178, 179	80	nd	nd	Yes	Yes	nd
Macrophage migration inhibitory factor	P34884	MIF	203	nd	nd	nd	Yes	nd	+
Serum albumin	P07724	ALBU	32	nd	nd	nd	nd	Yes	+/−
Mitochondrial proteins
Aconitate hydratase	Q99KI0	ACON	8, 9–11	17	Yes	nd	nd	Yes
ADP/ATP translocase 1, SLC25A4	P48962	ADT1	nd	55.57	nd	nd	nd	Yes
ADP/ATP translocase 2, SLC25A5	P51881	ADT2	nd	54.56	nd	nd	nd	nd
Aspartate aminotransferase	P05202	AATM	107–109	49	nd	nd	Yes	Yes
Aspartate glutamate carrier 1, SLC25A12	Q8BH59	CMC1	nd	76	nd	nd	nd	nd
ATP synthase F0 complex B	Q9CQQ7	AT5F1	nd	60	nd	nd	nd	nd
ATP synthase F0 complex D	Q9DCX2	ATP5H	157	nd	Yes	nd	nd	nd
ATP synthase F1 complex α	Q03265	ATPA	55–57	29	Yes	Yes	Yes	Yes
ATP synthase F1 complex β	P56480	ATPB	62, 63	nd	Yes	Yes	Yes	Yes
ATP synthase F1 complex γ	Q91VR2	ATPG	134	nd	nd	nd	Yes	Yes
ATP synthase O subunit	Q9DB20	ATPO	165	nd	nd	nd	nd	nd
Creatine kinase ubiquitous	P30275	KCRU	101	nd	Yes	Yes	nd	Yes
Cytochrome c oxidase Va	P12787	COX5A	167	nd	Yes	nd	nd	nd
Cytochrome c oxidase Vb	P19536	COX5B	201	nd	nd	nd	nd	nd
Cytochrome c oxidase Via	P43024	CX6A1	176	nd	nd	nd	nd	nd
Cytochrome c somatic	P62897	CYC	208	nd	nd	nd	Yes	Yes
Elongation factor Tu	Q8BFR5	EFTU	93	nd	nd	nd	nd	nd
Glucose regulated protein 75 kDa	P38647	GRP75	12	nd	Yes	nd	nd	nd
Hspd1, Hsp60	P63038	CH60	44	nd	Yes	Yes	nd	Yes
Isocitrate dehydrogenase 3 α	Q9D6R2	IDH3A	113, 115	nd	nd	nd	Yes	nd
Malate dehydrogenase, mitochondrial	P08249	MDHM	129.13	nd	Yes	nd	Yes	Yes
Mitochondrial inner membrane protein	Q8CAQ8	IMMT	3	74	nd	nd	nd	nd
NADH-ubiquinone oxidoreductase 24 kDa	Q9D6J6	NUHM	151	nd	nd	nd	nd	nd
NADH-ubiquinone oxidoreductase 30 kDa	Q9DCT2	NUGM	152	nd	nd	nd	nd	nd
NADH-ubiquinone oxidoreductase 42 kDa	Q99LC3	NUDM	117	nd	nd	nd	nd	nd
NADH-ubiquinone oxidoreductase 75 kDa	Q91VD9	NUAM	212	nd	nd	nd	nd	Yes
Oxoglutarate dehydrogenase	Q9D2G2	ODO2	68.72	nd	nd	nd	nd	nd
Phosphate carrier protein, Slc25A25	Q8VEM8	MPCP	nd	53	nd	nd	nd	nd
Prohibitin	P67778	PHB	146	56	nd	nd	nd	nd
Pyruvate dehydrogenase E1 α	P35486	ODPA	94	nd	nd	nd	nd	nd
Pyruvate dehydrogenase E2	NP663589	(NCBI)	217	nd	nd	nd	nd	nd
Pyruvate dehydrogenase E3	O08749	DLDH	51.52	nd	nd	nd	nd	nd
Succinate dehydrogenase subunit A	Q8K2B3	DHSA	34.35	nd	nd	nd	nd	nd
Succinyl-CoA ligase β	Q9Z2I9	SUCB1	81	nd	nd	nd	nd	nd
Superoxide dismutase 2, Mn	P09671	SODM	161	nd	Yes	nd	Yes	nd
Peroxide reductase	P20108	PRDX3	153	nd	nd	nd	nd	nd
Ubiquinol-cytochrome c reductase Fe-S	Q9CR68	UCRI	162	nd	nd	nd	nd	nd
Ubiquinol-cytochrome c reductase core 2	Q9DB77	UQCR2	195	47	nd	nd	Yes	Yes
Ubiquinol-cytochrome c reductase core 1	Q9CZ13	UQCR1	66.67	nd	Yes	nd	nd	Yes
Voltage-dependent anion channel 1	Q60932	VDAC1	132–144	52	Yes	nd	nd	Yes
Voltage-dependent anion channel 2	Q60930	VDAC2	143	nd	nd	nd	nd	Yes
			131 proteins	62	98	38	93	98

IEF spot number corresponds to spot number in Figure 1A; 16BAC spot number corresponds to spot number in Figure 1B. IEF, 2D-IEF/SDS-PAGE-MS; 16BAC, 16-BAC/SDS-PAGE-MS; LC, 2D-LC/LC-MS. IEF-T, Taylor et al. (2004); IEF-V and LC-V, Vanrobaeys et al. (2005); LC-R, Roth et al. (2006). Allen, Signal detected in white matter by in situ hybridization (Allen brain atlas available at www.brain-map.org). Bold, Proteins diminished in PLP^null myelin; nd, not detected.