Table 1.

Serotonin tissue content (picograms per microliter) and turnover (5-HIAA/5-HT)

Brain region	WT-saline		CREB^αΔ-saline		WT-PCPA		CREB^αΔ-PCPA
Brain region	Levels	Turnover	Levels	Turnover	Levels	Turnover	Levels	Turnover
Amygdala	367 ± 22	0.52 ± 0.03	338 ± 24	0.53 ± 0.03	62 ± 4	0.24 ± 0.01	57 ± 6	0.25 ± 0.02
Frontal cortex	223 ± 6	0.53 ± 0.02	196 ± 11	0.52 ± 0.02	54 ± 21	0.33 ± 0.13	34 ± 8	0.19 ± 0.03
Hippocampus	653 ± 59	1.07 ± 0.05	690 ± 59	1.14 ± 0.08	82 ± 7	0.36 ± 0.03	64 ± 9	0.32 ± 0.05
Nucleus accumbens	195 ± 11	0.52 ± 0.06	198 ± 35	0.42 ± 0.03	41 ± 7	0.23 ± 0.04	37 ± 9	0.18 ± 0.04
Brainstem	457 ± 36	1.04 ± 0.09	440 ± 38	1.02 ± 0.07	128 ± 22	0.92 ± 0.05	124 ± 16	0.69 ± 0.07

Mice (n = 8–9 per group) were killed 18 h after the last injection of PCPA. Brains were microdissected and HPLC coupled with EC was used to assay the filtered supernatant for serotonin content. Baseline serotonin levels, turnover, and depletion in CREB^αΔ mutant mice did not differ from that of WT mice throughout the amygdala, cortex, hippocampus, nucleus accumbens, and raphe.