Parameters for the flash responses of control and transgenic mouse rods
| rho+/− (n = 24) | S-opsinrho−/−(n = 20) | S-opsin-1D4rho−/−(n = 20) | S-opsinrho+/− (n = 13) | S-opsin-1D4rho+/−(n = 13) | |
|---|---|---|---|---|---|
| Dark current, Id (pA) | 19.1 ± 1.1 | 16.6 ± 1.8 | 15.6 ± 1.2 | 15.7 ± 0.9 | 19.1 ± 1.5 |
| Sensitivity at λmax′ S (pA photons−1/μm2) | 1.3 ± 0.2 × 10 − 1 (500 nm) | 7.4 ± 1.6 × 10 − 3 (360 nm) | 5.0 ± 0.5 × 10 − 3 (360 nm) | 0.7 ± 0.1 × 10 − 1 (500 nm) | 1.1 ± 0.1 × 10 − 1 (500 nm) |
| Single-photon response, a (pA) | 0.56 ± 0.04 | 0.30 ± 0.03 | 0.21 ± 0.01 | 0.43 ± 0.07 (500 nm) | 0.72 ± 0.07 (500 nm) |
| 0.42 ± 0.06 (360 nm) | 0.77 ± 0.06 (360 nm) | ||||
| Time to peak (ms) | 158 ± 5 | 153 ± 8 | 155 ± 9 | 122 ± 5 (500 nm) | 137 ± 2 (500 nm) |
| 122 ± 7 (360 nm) | 137 ± 2 (360 nm) | ||||
| Integration time (ms) | 254 ± 16 | 221 ± 16 | 204 ± 17 | 200 ± 12 (500 nm) | 224 ± 18 (500 nm) |
| 190 ± 14 (360 nm) | 216 ± 16 (360 nm) | ||||
| Normalized sensitivity at λmax, S/I d (photons−1/μm2) | 6.8 ± 0.7 × 10 − 3 (500 nm) | 5.1 ± 1.0 × 10 − 4 (360 nm) | 3.5 ± 0.4 × 10 − 4 (360 nm) | 4.2 ± 0.3 × 10 − 3 (500 nm) | 5.7 ± 0.6 × 10 − 3 (500 nm) |
| Dark current variance (pA2) | 0.11 ± 0.01 (n = 14) | 0.10 ± 0.01 (n = 14) | 0.08 ± 0.01 (n = 13) | 0.10 ± 0.01 (n = 11) | 0.11 ± 0.01 (n = 14) |
Values given are mean ± SEM.