Synaptic vesicles | Pool sizes | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Total volume | Putative RRP ≤60 nm | Putative RP 60–200 nm distance | Putative reserve pool >200 nm | ||||||||
No. | μm3 | % | No. | % | No./AZ | No. | % | No./AZ | No. | % | |
P28 | |||||||||||
1 | 11,018 | 0.42 | 7.1 | 351 | 3.2 | 18.47 | 1799 | 16.3 | 94.68 | 8868 | 80.5 |
2 | 25,683 | 0.83 | 10.2 | 1152 | 4.5 | 31.13 | 4624 | 18.0 | 124.97 | 19,907 | 77.5 |
3 | 15,198 | 0.07 | 1.4 | 636 | 4.2 | 35.33 | 3350 | 22.0 | 186.11 | 11,212 | 73.8 |
7 | 48,633 | 0.63 | 6.9 | 2777 | 5.7 | 61.71 | 13,139 | 27.0 | 291.97 | 32,717 | 67.3 |
Mean | 25,133 | 0.49 | 6.4 | 1229 | 4.4 | 36.66 | 5728 | 20.8 | 174.43 | 18,176 | 74.8 |
CV | 0.67 | 0.6 | 0.88 | 0.89 | 0.59 | ||||||
Adult | |||||||||||
1 | 13,269 | 0.31 | 2.4 | 432 | 3.3 | 12.71 | 2323 | 17.5 | 70.67 | 10,434 | 78.6 |
3 | 17,138 | 0.36 | 6.6 | 599 | 3.5 | 42.78 | 3531 | 20.6 | 263.71 | 12,847 | 75.0 |
4 | 25,028 | 0.25 | 2.0 | 1034 | 4.1 | 57.44 | 4723 | 18.9 | 276.83 | 19,011 | 76.0 |
7 | 7559 | 0.13 | 5.6 | 367 | 4.9 | 52.42 | 1531 | 20.3 | 233.14 | 5560 | 73.6 |
Mean | 15,749 | 0.26 | 4.2 | 608 | 4.0 | 41.34 | 3720 | 19.3 | 211.09 | 11,963 | 75.8 |
CV | 0.38 | 0.6 | 0.49 | 0.46 | 0.47 |
Summary of structural parameters relevant for release that have been extracted from the detailed 3-D reconstructions of MFBs in P28 and adult rats. For the RRP and RP, two values are given: the total number of synaptic vesicles per MFB and per individual AZ. For the reserve pool, the number of synaptic vesicles per bouton is given.