Vehicle | 8-Br-cGMP | SB203580 | 8-Br-cGMP + SB203580 | |
---|---|---|---|---|
Wild type | 100 ± 4 | 129 ± 3 | 102 ± 2 | 126 ± 3 |
T276D | 131 ± 3 | 127 ± 2 | 130 ± 1 | 130 ± 1 |
I425V | 130 ± 4 | 130 ± 3 | 125 ± 3 | 126 ± 5 |
Cells expressing wild-type hSERT, T276D, or I425V mutant were treated with SB203580 (10 μm in 0.1% DMSO) for 10 min, 8-Br-cGMP (100 μm) for 30 min, or preincubated with SB203580 (10 μm) for 10 min before adding 100 μm 8-Br-cGMP for 30 min before transport assay. Transport was measured in a 10 min incubation with [3H]5-HT (20 nm), as described in Materials and Methods. Data are means ± SD from six measurements in two separate experiments as the percentage of transport activity relative to wild type treated with vehicle (0.1% DMSO).