List of oligonucleotides used for RT-PCR analyses of human circumvallate papillae cDNA
| Oligonucleotide | Sequence (5′ to 3′) | Ann. | Amplicon size |
|---|---|---|---|
| hTAS2R1_for | TGTGGTGGTGAATGGCATTG | 62°C | 790 bp |
| hTAS2R1_rev | CAGCACTTACTGTGGAGGAGGAAC | ||
| hTAS2R3_for | ACACATGATTCAGGGATAATAATGCAAA | 60°C | 697 bp |
| hTAS2R3_rev | TTAGCCATCTTGGTTTTTGGTAGGAAATT | ||
| hTAS2R4_for | TACAGTGGTCAATTGCAAAACTTGG | 62°C | 726 bp |
| hTAS2R4_rev | AATGTCCTGGAGAGTAAAGGGTGG | ||
| hTAS2R5_for | TGGTCCTCATATAACCTCATTATCCTGG | 63°C | 668 bp |
| hTAS2R5_rev | CTGCCATGAGTGTCTCCCA | ||
| hTAS2R7_for | TGTTTTATATTGGTGCTATATCCAGATGTCTATGC | 64°C | 664 bp |
| hTAS2R7_rev | GGATAAATGAATGACTTGAGGGGTAGATTAGAG | ||
| hTAS2R8_for | CAATTTAGTTATCGCCAGAATTTGTTTGATC | 64°C | 722 bp |
| hTAS2R8_rev | TTATTTAAAACAATTAAAATAAGTGAGTGACCCAAGG | ||
| hTAS2R9_for | TGAATTGACCATAGGGATTTGGG | 62°C | 807 bp |
| hTAS2R9_rev | ATAATTAGAATGAATGAATGGCTTGATGG | ||
| hTAS2R10_for | GACTTGTAAACTGCATTGACTGTGCC | 62°C | 784 bp |
| hTAS2R10_rev | AAAGAGGCTTGCTTTAGCTTGCTG | ||
| hTAS2R13_for | GGGTCAGTAAAAGAGAGCTGTCCTC | 61°C | 743 bp |
| hTAS2R13_rev | ATCAGAAGAAAGGAGTGGCTTGAAG | ||
| hTAS2R14_for | GCTTTGGCAATCTCTCGAATTAGC | 60°C | 771 bp |
| hTAS2R14_rev | CTCTAAATTCTTTGTGACCTGAGGGC | ||
| hTAS2R16_for | CCTGGGAATTTTTTAATATCCTTACATTCTGGT | 63°C | 449 bp |
| hTAS2R16_rev | GAAGCGCGCTTTCATGCTT | ||
| hTAS2R38_for | ACAGTGATTGTGTGCTGCTG | 58°C | 766 bp |
| hTAS2R38_rev | GCTCTCCTCAACTTGGCATT | ||
| hTAS2R39_for | TGTCGCCATTTCTCATCACCTTA | 62°C | 849 bp |
| hTAS2R39_rev | ATTGAGTGGCTGGCAGGGTAG | ||
| hTAS2R40_for | AGAGTGCATCACTGGCATCCTT | 60°C | 685 bp |
| hTAS2R40_rev | GAGGATGAGAAAGTAGCTGGTGGC | ||
| hTAS2R41_for | GGTTGCTGCCCTTGGATATGA | 63°C | 740 bp |
| hTAS2R41_rev | TGAAGATGAGGATGAAGGGATGG | ||
| hTAS2R42_for | ATGGCCACCGAATTGGACA | 62°C | 850 bp |
| hTAS2R42_rev | GCTTGCTGTTTCCCAGAATGAG | ||
| hTAS2R43_for | GCTAATGGCTTCATAGCAC | 52°C | 589 bp |
| hTAS2R43_rev | GATCTTGAGATCCTTTACCAC | ||
| hTAS2R44_for | CATTGGTAAATTCCATTGAGC | 53°C | 653 bp |
| hTAS2R44_rev | GATATCATTATGGACAGAAAGTAAAC | ||
| hTAS2R45_for | CTCCTTTGCTGACCAAATTGTC | 58°C | 710 bp |
| hTAS2R45_rev | GAACGGGTGGGCTGAAGAAC | ||
| hTAS2R46_for | GAGTTGAATCCAGCTTTTAAC | 52°C | 607 bp |
| hTAS2R46_rev | ATAGCTGAATGCAATAGCTTC | ||
| hTAS2R47_for | GGTGTTATTACTACATTGGTATGCAACTC | 59°C | 604 bp |
| hTAS2R47_rev | AAGACAGGTTGCTTTTCCAGC | ||
| hTAS2R48_for | GGTTTACTCTGGGTCATGTTATTC | 56°C | 607 bp |
| hTAS2R48_rev | TTTGCTCTGCTGTGTCCTAAG | ||
| hTAS2R49_for | GCACTGATAAATTTCATTGCCTGG | 62°C | 786 bp |
| hTAS2R49_rev | TTGTTCCCCCAAATCAGAATGAAT | ||
| hTAS2R50_for | GGTAAATTTCATTGACTGGGTGAAGAG | 62°C | 710 bp |
| hTAS2R50_rev | CCTTGCTAACCATGACAACTGGG | ||
| hTAS2R60_for | CAGGCAATGGCTTCATCACTG | 63°C | 748 bp |
| hTAS2R60_rev | TCCCACACCCAGAATTTAAAGTCC |
The oligonucleotide sequences, the annealing temperatures used for amplification reactions (Ann.), and the corresponding amplicon sizes are given. for, Forward; rev, reverse.