PCR oligo primers and sequence specific hybridization FRET probes for ephrin As and housekeeping gene HPRT designed and synthesized by Proligo
| Oligo name | GenBank id | Sequence: (5′-3′) | Size | Position | |
|---|---|---|---|---|---|
| 1 | ephrinA1_forward | gi 12805174 gb BC002046.1 | 5′-TCATgCggTTggACAC-3′ | 16 | 994-1009 |
| 2 | ephrinA1_reverse | gi 12805174 gb BC002046.1 | 5′-CCgggCTACAAACCTC-3′ | 16 | 1172-1187 |
| 3 | ephrinA1_probeA | gi 12805174 gb BC002046.1 | 5′-AgCCAggTACAgAAgAggC(Fluo)-3′ | 19 | 1100-1118 |
| 4 | ephrinA1_probeB | gi 12805174 gb BC002046.1 | 5′-(Red640)CTTgggCTgACCCAgCA(Phosphate)-3′ | 17 | 1121-1137 |
| 5 | ephrinA2_forward | gi 29145116 gb BC048697.1 | 5′-AggTTTATgTgCgTCCA-3′ | 17 | 509-525 |
| 6 | ephrinA2_reverse | gi 29145116 gb BC048697.1 | 5′-gTCAgAgggCAAAggT-3′ | 16 | 691-706 |
| 7 | ephrinA2_probeA | gi 29145116 gb BC048697.1 | 5′-CACCgTCCCTgTgCTgT(Fluo)-3′ | 17 | 615-631 |
| 8 | ephrinA2_probeB | gi 29145116 gb BC048697.1 | 5′-(Red640)TCCCTTCTgggCTCCTAgT(Phosphate)-3′ | 19 | 634-652 |
| 9 | ephrinA3_forward | gi 12861020 dbj AK020438.1 | 5′-gATTgAATCCAAgTTTACAAATAgAC-3′ | 26 | 633-658 |
| 10 | ephrinA3_reverse | gi 12861020 dbj AK020438.1 | 5′-TgTgCgTTgCATAggT-3′ | 16 | 885-900 |
| 11 | ephrinA3_probeA | gi 12861020 dbj AK020438.1 | 5′-ACAggCAgAgTCCCTTCC(Fluo)-3′ | 18 | 751-768 |
| 12 | ephrinA3_probeB | gi 12861020 dbj AK020438.1 | 5′-(Red640)TTCTTAACCTTgACCTTTCTCgACTTCC(Phosphate)-3′ | 28 | 771-798 |
| 13 | ephrinA4_forward | gi 12848791 dbj AK012195.1 | 5′-gAgAAAgCggTACgTCC-3′ gi 12848791 dbj AK012195.1 | 17 | 600-616 |
| 14 | ephrinA4_reverse | gi 12848791 dbj AK012195.1 | 5′-ggCATCgCCTgTATgg-3′ | 16 | 779-794 |
| 15 | ephrinA4_probeA | gi 12848791 dbj AK012195.1 | 5′-CTgCTCCCAATCCTCCgT(Fluo)-3′ | 18 | 668-685 |
| 16 | ephrinA4_probeB | gi 12848791 dbj AK012195.1 | 5′-(Red640)CCTgAgAgTTCTgTgAgCCA(Phosphate)-3′ | 20 | 688-707 |
| 17 | ephrinA5_forward | gi 25955544 gb BC040218.1 | 5′-ATAgCTTTggCTACTCCT-3′ | 18 | 1853-1870 |
| 18 | ephrinA5_reverse | gi 25955544 gb BC040218.1 | 5′-CTgACTCTCCTggTgAT-3′ | 17 | 2030-2046 |
| 19 | ephrinA5_probeA | gi 25955544 gb BC040218.1 | 5′-gTggCCTTTCCCCAATTCCC(Fluo)-3′ | 20 | 1948-1967 |
| 20 | ephrinA5_probeB | gi 25955544 gb BC040218.1 | 5′-(Red640)CCCgATACCCACgTTCTgC(Phosphate)-3′ | 19 | 1970-1988 |
| 21 | RatHPRT_forward | gi 70778838 ref NM_012583.2 | 5′-CCAgCgTCgTgATTAgT-3′ | 17 | 83-99 |
| 22 | RatHPRT _reverse | gi 70778838 ref NM_012583.2 | 5′-ACACAgAgggCCACAA-3′ | 16 | 251-266 |
| 23 | RatHPRT _probeA | gi 70778838 ref NM_012583.2 | 5′-ATTTggAAAAggTgTTTATTCCTCATggAC(Fluo)-3′ | 30 | 158-187 |
| 24 | RatHPRT _probeB | gi 70778838 ref NM_012583.2 | 5′-(Red640)ATTATggACAggACTgAAAgACTTgC(Phosphate)-3′ | 26 | 190-215 |