Effect of carbachol on the properties of GHRH-GFP neurons
| Infrathreshold properties (% over control)a | Males | Females | ||
|---|---|---|---|---|
| Slope | Intercept | Slope | Intercept | |
| Carbachol 10 μm | −1.4 ± 3.4 (5) | −4.7 ± 3.3 (5) | −6.0 ± 11.3 (3) | +5.0 ± 8.4 (3) |
| Carbachol 100 μm | −4.3 ± 6.4 (5) | −5.4 ± 8.9 (5) | ||
| TTX 500 nm | −5.6 ± 3.6 (7) | −4.4 ± 3.8 (7) | −1.1 ± 4.7 (6) | −0.7 ± 6.8 (6) |
| TTX plus carbachol 10 μm | −4.1 ± 5.5 (6) | −1.9 ± 4.4 (6) | −0.9 ± 9.4 (6) | −2.6 ± 12.4 (6) |
| TTX plus carbachol 100 μm | −8.7 ± 6.2 (5) | −2.2 ± 4.5 (5) | ||
The number of observations is indicated in parentheses.
↵aInfrathreshold current–voltage relationships were performed as stated in Materials and Methods, except that 50-ms-duration pulses were performed.