Percentage of TRPV4-expressing cells in each neural type
| Pyramidal neurons | Interneurons | Granule neurons | |
|---|---|---|---|
| Calcium imaging by heat application (in wild-type neurons) | 186/347 (53.6%) | 124/254 (48.8%) | 105/211 (49.8%) |
| Staining (TRPV4 mRNA + GAD65 staining) | 88/136 (64.3%) | 64/112 (57.1%) | 74/138 (53.6%) |
The TRPV4-expressing ratio was represented as the responded or TRPV4-positive cells/total cell numbers of each cell type. The pyramidal neurons were identified by morphology and as GAD65-negative neurons.