Table 1.

Activation of TRPV2, TRPA1, and TRPV1

Compound	Rat TRPV2		Rat TRPA1		Rat TRPV1
Compound	% Response at 100 μm	EC₅₀ (μm)	% Response at 100 μm	EC₅₀ (μm)	% Response at 100 μm	EC₅₀ (μm)
2-APB (15 μm)^a	100	10.0 ± 2.8			64	33.6
Mustard oil (50 μm)	8		100	7.1	22
Capsaicin (500 nm)					100	0.02
Δ⁹-THC	98	15.5	62	32.3	0
Cannabidiol	160	3.7	100	81.4	21
Cannabinol	67	77.7	22		0
11-hydroxy-Δ⁹-THC	57		2		24
O-1821^a	93		7		15
O-1918	6		0		14
CAY 10429	0		86	9.0	1
Nabilone	58		4		26
CP55940	42		48		0
HU-210	38		1
HU-211	30		0		1
HU-308	10		12		23
HU-331 (50 μm)^a	46		95	3.2	37
WIN 55212–2^a	0		82	9.8	40
WIN 55212–3^a	7		95	21.7	43
JWH-015	14		22		58	41.2
JWH-133	4				0
Anandamide	0		0		92	6.9
2-AG	29		0		61	8.4
Arvanil	11				113	0.02
AM404	6		0		104	0.47

The percentage responses were normalized to 15 μm 2-APB (for TRPV2), 50 μm mustard oil (for TRPA1), and 0.5 μm capsaicin (for TRPV1).
↵^aA response was observed in HEK293 parental cells, which was subtracted.