Summary of the responsiveness of hTRPA1, hTRPA1-dTM5, and hTRPA1-S873V/T874L to TRPA1 modulators
| Compound | Effect | hTRPA1 EC50 or IC50 (μm) | hTRPA1-dTM5 EC50 or IC50 (μm) | hTRPA1-S873V/T874L EC50 or IC50 (μm) |
|---|---|---|---|---|
| Menthol | Activation | 28.4 ± 3.6 | No activation | 161.2 ± 31.5 |
| Thymol | Activation | 62.5 ± 3.2 | No activation | >400a |
| FTS | Activation | 7.3 ± 1.1 | No activation | 11.8 ± 8.3 |
| Trinitrophenol | Activation | 383.8 ± 5.7 | 367.9 ± 76.7 | 218.6 ± 3.6 |
| MO | Activation | 2.33 ± 0.74 | 1.32 ± 0.46 | 0.98 ± 0.24 |
| AP18 | Inhibition | 0.60 ± 0.17 | No inhibition | No inhibition |
| AMG5445 | Inhibition | 2.23 ± 0.58 | No inhibition | No inhibition |
| HCO30031 | Inhibition | 12.87 ± 2.67 | 11.47 ± 2.39 | 8.51 ± 1.36 |
| Ruthenium red | Inhibition | 1.5 ± 0.2 | 0.48 ± 0.03 | 0.64 ± 0.13 |
↵aCould not be accurately fit with a Hill Equation.