Antibodies used for Western blotting and immunohistochemistry
| Antibody | Type | Species | Epitope; designation | Dilution (μg/ml) | Source |
|---|---|---|---|---|---|
| Cx36 | Polyclonal | Rabbit | C terminus; 36-4600 | 1 | Invitrogen |
| Cx36 | Polyclonal | Rabbit | Midregion; 51-6300 | 4 | Invitrogen |
| Cx36 | Monoclonal | Mouse | Midregion; 37-4600 | 3 | Invitrogen |
| Cx36 | Monoclonal | Mouse | C terminus; 39-4200 | 4 | Invitrogen |
| Cx43 | Polyclonal | Rabbit | C terminus; 71-0700 | 2 | Invitrogen |
| Cx45 | Polyclonal | Rabbit | C terminus; 40-7000 | 2 | Invitrogen |
| Cx45 | Monoclonal | Mouse | C terminus; 41-1700 | 2 | Invitrogen |
| Cx45 | Monoclonal | Mouse | aa 354–367; MAB3101 | 4 | Millipore Bioscience Research Reagents |
| ZO-1 | Polyclonal | Rabbit | aa 463–1109; 61-7300 | 1 | Invitrogen |
| ZO-1 | Monoclonal | Mouse | aa 334–634; 33-9100 | 4 | Invitrogen |
| ZO-2 | Monoclonal | Mouse | aa 87–208; Ab611561 | 3 | BD Biosciences |
| ZONAB | Polyclonal | Rabbit | C terminus; 40-2800 | 2 | Invitrogen |
| GST | Polyclonal | Rabbit | Whole protein; Ab06-332 | 1 | Millipore |
aa, Amino acids.