Single-channel properties for wild-type and T686A GluR2 channels
| GluR2-wt | T686A | |||
|---|---|---|---|---|
| Glutamate | Quisqualate | Glutamate | Quisqualate | |
| Conductance (pS) | ||||
| O1 | 6.5 ± 0.2 | 7.5 ± 0.2 | 6.1 ± 0.2 | 6.4 ± 0.4 |
| O2 | 14.8 ± 0.2 | 15.4 ± 0.3 | 14.5 ± 0.2 | 14.7 ± 0.2 |
| O3 | 23.7 ± 0.4 | 24.2 ± 0.3 | 23.5 ± 0.3 | 24.0 ± 0.5 |
| O4 | 36.2 ± 0.7 | 36.8 ± 0.2 | 36.9 ± 0.7 | 36.6 ± 0.6 |
| Relative occupancy (%) | ||||
| O1 | 46.7 ± 3.3A | 35.6 ± 8.9a | 71.5 ± 2.2 | 55.4 ± 6.0a |
| O2 | 32.4 ± 2.5a | 33.8 ± 3.1a | 23.4 ± 2.3 | 27.3 ± 1.1 |
| O3 | 19.1 ± 1.5A | 27.3 ± 7.1a | 4.7 ± 0.8 | 15.1 ± 4.1a |
| O4 | 2.0 ± 0.4A | 4.0 ± 2.2 | 0.4 ± 0.2 | 2.3 ± 1.2 |
| Shut-time distributions | ||||
| τ 1 (ms) | 0.4 ± 0.1A | 0.3 ± 0.1A,B | 2.1 ± 0.1 | 1.2 ± 0.3a |
| area (%) | (30 ± 2.9) | (28 ± 3.4) | (45.7 ± 5.5) | (32 ± 4.2) |
| τ 2 (ms) | 3.6 ± 0.5A | 2.7 ± 0.5a | 18.3 ± 4.4 | 8.1 ± 2.8 |
| area (%) | (22 ± 2.9) | (19 ± 2.9) | (24 ± 1.2) | (27 ± 1.9) |
| τ 3 (ms) | 60.9 ± 11 | 57 ± 11 | 155 ± 35 | 73 ± 13 |
| area (%) | (21 ± 3.3) | (27 ± 5.6) | (25 ± 5.3) | (24 ± 4.5) |
| τ 4 (ms) | 396 ± 85 | 384 ± 66 | 729 ± 98 | 459 ± 155 |
| area (%) | (27 ± 3.5) | (29 ± 4.3) | (5.7 ± 0.9) | (16 ± 2.6) |
Single-channel currents were recorded in outside-out patches in response to glutamate (10 mm for GluR2-wt, 50 mm for T686A) or quisqualate (0.5–1 mm for GluR2-wt, 5 mm for T686A). Mean ± SEM values from four to six patches. If F ratio tests indicated the results were homogeneous with respect to variance, comparisons of the mean values in each row were made with one-way ANOVA. When this was not the case, pairwise comparisons were made with two-tailed Student's t test assuming unequal variance. Lowercase and uppercase superscripts indicate significance at p < 0.05 and 0.01, respectively.
↵a,ADifferent from corresponding T686A results with glutamate.
↵b,BDifferent from corresponding T686A results with quisqualate.