No. | Reaction | Mean value | SDs | Search range | Result range |
---|---|---|---|---|---|
0′-1a | CaM + Munc13 ⇆ CaM-Munc13 | [Munc13] = 1.8e-8 | 1.1e-8 | 1e-10 to 1e-7 | 1e-7 to 4.5e-7 |
kon = 1.3e4 | 7.5e3 | 1e1 to 1e9 | 1.3e3 to 2.1e4 | ||
Kd = 2.5e-6 | 2.2e-6 | 1e-10 to 1e-5 | 9e-7 to 8e-6 | ||
0′-1b | CaM–Munc13 + Ca2+ ⇆ CaM–Munc13′ | [CaM] = 1e-6 | No search | No search | No search |
(in excess) | |||||
kon = 6.35 1e8 | 2.5e9 | 1e6 to 1e10 | 3e6 to 1e10 | ||
Kd = 2.5e-7 | 1e-7 | 1e-14 to 1e-5 | 5e-8 to 5e-7 | ||
0′-2 | CaM–Munc13′ + Ca2+ ⇆ CaM–Munc13″ | kon = 6.4e6 | 5e7 | 1e6 to 1e9 | 5.3e6 to 7.4e6 |
Kd = 7e-6 | 3e-7 | 1e-8 to 1e-5 | 1e-6 to 1e-5 | ||
0′- 3 | CaM–Munc13″ + Ca2+ ⇆ CaM–Munc13‴ | kon = 2.6e8 | 3.2e8 | 1e6 to 1e9 | 1e7 to 1e9 |
Kd = 5.6e-6 | 2.5e-6 | 1e-8 to 1e-5 | 2e-6 to 1e-5 | ||
3a | CaM–Munc13‴ + SNARE ⇆ CaM–Munc13–SNARE (= SNARE′) | kon = 9.5e10 | 1. e10 | 1e6 to 1e11 | 5e10 to 1e11 |
Kd = 3e-9 | 1.5e-9 | 1e-13 to 1e-7 | 9e-10 to 5e-9 |
The parameters are results of the genetic algorithm searching method. For each parameter, we present the mean value, SDs, the search range, and the result range.