Virgin rats were treated with AP (3 and 1 mg/kg, s.c.) or veh at t = −20 and −2 h and then killed by conscious decapitation at t = 0 h (n = 6–7 rats per group).
| mRNA | Brain region | Vehicle | AP | ||
|---|---|---|---|---|---|
| # Positive cells | Grain density per cell (μm2) | # Positive cells | Grain density per cell (μm2) | ||
| pENK-A | NTS A2 | 115 ± 3 | 34.0 ± 0.3 | 141 ± 10* | 38.4 ± 0.9** |
| pENK-A | mgPVN | 21.8 ± 4.9 | 45.1 ± 1.0 | 17.5 ± 3.6 | 45.6 ± 1.0 |
| pENK-A | Arc | 46.5 ± 4.5 | 30.6 ± 0.5 | 51.2 ± 1.3 | 30.9 ± 0.4 |
| POMC | Arc | 46.8 ± 2.7 | 19.0 ± 0.4 | 47.0 ± 1.9 | 18.9 ± 0.4 |
| MOR | NTS A2 | 117.1 ± 12.2 | 19.2 ± 0.6 | 108.5 ± 7.5 | 18.6 ± 0.7 |
| CRH | pPVN | 131.1 ± 11.7 | 49.3 ± 6.6 | 131.1 ± 6.9 | 44.6 ± 3.5 |
mg PVN, Magnocellular subdivision of the paraventricular nucleus; Arc, arcuate nucleus.
↵*p < 0.02, **p < 0.001 versus veh group. Values are mean ± SEM.