Resting membrane potential and input resistance of astrocytes in situ under control conditions and after adding Ba2+
| Age | Resting potential (mV) | Input resistance (MΩ) |
|---|---|---|
| P9, control | −71.9 ± 3.4 (n = 10) | 38.7 ± 17.0 (n = 10) |
| P9, 100 μm Cbx | −71.9 ± 1.6 (n = 6) | 44.2 ± 17.5 (n = 6) |
| P9, 100 μm Cbx, 100 μm BaCl2 | −61.0 ± 4.1a (n = 6) | 93.3 ± 49.8a (n = 6) |
| P9, Kir4.1−/− | −39.1 ± 9.8b (n = 9) | 143.0 ± 82.4b (n = 9) |
| P9, Kir4.1−/−, 100 μm BaCl2 | −38.3 ± 10.1 (n = 9) | 140.5 ± 54.3 (n = 9) |
Data were obtained from hGFAF/EGFP mice and from Kir4.1−/− mice crossed with hGFAP/EGFP mice. Cell numbers are given in parentheses.
↵aIn the presence of Cbx (100 μm; 30 min preincubation), Ba2+ significantly affected resting potential and input resistance.
↵bIn addition, resting potential and input resistance of astrocytes in control versus Kir4.1−/− mice differed significantly.