ACh response and Mor potentiation characteristics
| ACh response | Mor potentiation | ||||||
|---|---|---|---|---|---|---|---|
| EC50 (μm) | nH | IACh+Mor/IACha | % Change after MTSb | ||||
| α3β2 wt | 60 ± 6 | 0.82 ± 0.06 | (3; 1) | 1.9 ± 0.1 | (19; 4) | 3 ± 3 | (5; 1) |
| α3T115Cβ2 | 240 ± 30 | 0.96 ± 0.09 | (7; 2) | 2.3 ± 0.3 | (25; 4) | −30 ± 4* | (8; 2) |
| α3β2T150C | 20 ± 1 | 0.84 ± 0.06 | (3; 1) | 1.6 ± 0.1 | (29; 3) | 52 ± 17* | (5; 1) |
| α3β2S148C | 66 ± 9 | 0.79 ± 0.08 | (5; 1) | 4.9 ± 1.0 | (5; 1) | −7 ± 10 | (5; 1) |
| α3β2S192C | 30 ± 4 | 0.57 ± 0.05 | (8; 1) | 1.4 ± 0.1 | (13; 3) | 19 ± 5* | (5; 1) |
| α3β2T193C | 24 ± 3 | 0.74 ± 0.07 | (5; 1) | 1.5 ± 0.1 | (12; 1) | 2 ± 4 | (4; 1) |
| α3A106Cβ2 | 61 ± 7 | 0.64 ± 0.04 | (6; 1) | 1.7 ± 0.2 | (5; 1) | 27 ± 10* | (5; 1) |
| α3S161Cβ2 | 190 ± 20 | 0.77 ± 0.07 | (6; 1) | 1.3 ± 0.1 | (5; 1) | 3 ± 8 | (4; 1) |
Values are means ± SEM. In each experiment, the number of oocytes tested and the number of donors, respectively, are given in parentheses. wt, Wild type.
↵aThe standard measure of potentiation used [ACh] ∼ EC50 ± 10 μm Mor.
↵bAll experiments used 100 μm MTSET. Treatment involved coapplying with ACh at the control concentration, except for α3β2T150C, α3β2S148C, and α3S161Cβ2, where the reagent was applied alone. Asterisks indicate the change in potentiation after treatment was statistically significant (p < 0.05) as measured by a paired comparison t test.