Immunophenotype of CD82–IRES–EGFP+, shRNA–ZsGreen+, and control cells at 7 and 90 dpi
| Region | Immunophenotype | CD82–IRES–EGFP+ | Control EGFP+ | shRNA–ZsGreen+ | Control ZsGreen+ | ||||
|---|---|---|---|---|---|---|---|---|---|
| 7 dpi | 90 dpi | 7 dpi | 90 dpi | 7 dpi | 90 dpi | 7 dpi | 90 dpi | ||
| White matter | CC1+ | 77 of 79 (97.5%) | 67 of 67 (100%) | 30 of 68 (44.1%) | 44 of 57 (77.2%) | 13 of 58 (22.4%) | 7 of 57 (12.3%) | 24 of 55 (43.6%) | 38 of 53 (71.7%) |
| MBP+ | 65 of 87 (74.7%) | 59 of 59 (100%) | 26 of 71 (36.6%) | 41 of 55 (74.5%) | 0 (0%) | 0 (0%) | 19 of 54 (35.2%) | 34 of 51 (66.7%) | |
| NG2+ | 0 (0%) | 0 (0%) | 25 of 55 (45.5%) | 7 of 51 (13.7%) | 40 of 66 (60.6%) | 53 of 65 (81.5%) | 25 of 56 (44.6%) | 9 of 57 (15.8%) | |
| PDGFRα+ | 0 (0%) | 0 (0%) | 27 of 62 (43.5%) | 6 of 49 (12.2%) | 39 of 67 (58.2%) | 30 of 56 (53.6%) | 26 of 60 (43.3%) | 8 of 56 (14.3%) | |
| olig2+ | 0 (0%) | 0 (0%) | 39 of 67 (58.2%) | 13 of 53 (24.5%) | 44 of 59 (74.6%) | 59 of 68 (86.8%) | 38 of 64 (59.4%) | 16 of 61 (26.2%) | |
| Cortex | GFAP+ | 0 (0%) | 11 of 49 (22.4%) | 12 of 53 (22.6%) | 29 of 45 (64.4%) | 47 of 56 (83.9%) | 67 of 72 (93.1%) | 13 of 55 (23.6%) | 31 of 47 (65.6%) |
| zebrinII+ | 62 of 62 (100%) | 43 of 46 (93.5%) | 29 of 65 (44.6%) | 16 of 47 (34.1%) | 0 (0%) | 0 (0%) | 21 of 48 (43.8%) | 14 of 45 (31.1%) | |
| PDGFRα+ | 0 (0%) | 0 (0%) | 15 of 53 (28.3%) | 6 of 48 (12.5%) | 19 of 54 (35.2%) | 9 of 66 (13.6%) | 16 of 46 (34.8%) | 6 of 42 (14.3%) | |
Five representative 40× fields from three brains were photographed per marker, and green and double-positive cells were manually counted. The percentage of double-positive cells was calculated by dividing the number of double-positive cells by the total number of green cells counted for each marker, separately. There are statistically significant differences between the distributions of all CD82–IRES–EGFP+/marker+ and control EGFP+/marker+ cells, almost all shRNA-ZsGreen+/marker+, and control ZsGreen+/marker+ cells, with the exception of PDGFRα+ in the cortex. There is no significant difference between the distributions of markers of control EGFP+ and control ZsGreen+ cells. CD82–IRES–EGFP+/control EGFP+: xCC1+2 = 2.924e−17; xMBP+2 = 3.494e−12; xNG2+2 = 1.424e−14; xPDGFRα+wm2 = 8.442e−14; xolig2+2 = 9.55e−20; xGFAP+2 = 1.544e−12; xzebrinII+2 = 2.343e−39; xPDGFRα+ctx2 = 1.686e−10 (x0.052 = 0.004, df = 1). shRNA–ZsGreen+/control ZsGreen+: xCC1+2 = 1.212e−14; xMBP+2 = 5.84e−24; xNG2+2 = 1.281e−62; xPDGFRα+wm2 = 2.017e−26; xolig2+2 = 3.454e−33; xGFAP+2 = 6.765e−38; xzebrinII+2 = 4.952e−18; xPDGFRα+ctx2 = 0.844 (x0.052 = 0.004, df = 1). Control EGFP+/control ZsGreen+: xCC1+2 = 0.513; xMBP+2 = 0.325; xNG2+2 = 0.586; xPDGFRα+wm2 = 0.578; xolig2+2 = 0.714; xGFAP+2 = 0.799; xzebrinII+2 = 0.581; xPDGFRα+ctx2 = 0.230 (x0.052 = 0.004, df = 1).