mRNA fold changes in spinal cord of C57BL/6 mice injected with CFA, CFA plus pertussis toxin (PTx), or PTx alone
| 3 h postinjection | 24 h postinjection | 48 h postinjection | 72 h postinjection | 96 h postinjection | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CFA | CFA + PTx | PTx | CFA | CFA + PTx | PTx | CFA | CFA + PTx | PTx | CFA | CFA + PTx | PTx | CFA | CFA + PTx | PTx | |
| MPO | 2.1 | 0.7 | 0.5 | 4.1 | 0.9 | 0.5 | 1.3 | 0.5 | 0.7 | 1.3 | 1.1 | 0.4 | 15.9 | 1.0 | 0.9 |
| COX2 | 2.6 | 5.9 | 0.9 | 3.5 | 3.9 | 0.7 | 5.5 | 3.8 | 1.0 | 2.9 | 2.1 | 0.9 | 5.5 | 4.4 | 0.8 |
| Mincle | 1.0 | 2.2 | 0.4 | 1.8 | 2.1 | 0.4 | 2.4 | 1.6 | 0.6 | 1.4 | 1.5 | 0.3 | 4.0 | 4.0 | 0.6 |
| IL1β | 1.1 | 1.2 | 0.4 | 0.9 | 0.8 | 0.4 | 0.8 | 1.1 | 0.4 | 1.1 | 1.1 | 0.4 | 2.3 | 3.1 | 0.8 |
| CXCL1 | 4.3 | 34.9 | 0.4 | 3.2 | 2.3 | 0.6 | 15.4 | 4.4 | 1.5 | 5.0 | 1.7 | 0.7 | 8.3 | 10.0 | 0.8 |
| CD45 | 1.5 | 1.5 | 1.2 | 2.4 | 1.9 | 1.3 | 2.1 | 1.7 | 1.3 | 1.6 | 1.8 | 1.1 | 2.7 | 2.1 | 1.3 |
mRNAs tested but showing no change in spinal cords of mice given CFA, CFA plus PTx, or PTx alone: HMGB1, NF-kb1, CX3CR1, CX3CL1, C5, NF-IL6, and CXCL9. Mice were sacrificed at the indicated times postinjection with CFA, CFA plus pertussis toxin, or pertussis toxin alone. Each determination employed three mice, and three determinations were made at each time point. Data are presented as means of fold differences over uninjected control mice. Boldface indicates that mRNA levels were at least 1.5-fold higher, and significantly different, than in the control samples at that time-point, with p < 0.05 (Mann–Whitney U test).