CO stripes and tracers injected | Case no. | Interinjection distance (μm)a | Label overlap (%)b | No. of single-labeled cells | No. of double-labeled cells | % Double-labeled cells | Figure | |||
---|---|---|---|---|---|---|---|---|---|---|
Stripe 1 Tracer 1 | Stripe 2 Tracer 2 | Tracer 1 | Tracer 2 | % of total labeled cellsc | % of smallest no. of cells labeled with one tracerd | |||||
Thick CTBg | Palelat CTB 488 | 260-m | −104 | 78.2 | 760 | 1034 | 116 | 6.1 | 13.2 | Fig. 8A–D |
Thick CTB 488 | Palelat CTB 555 | 308-m | 288 | 60.6 | 986 | 1351 | 193 | 7.6 | 16.4 | Supplemental Fig. 3e |
Thin CTBg | Palelat CTB 488 | 274-m | −184 | 89 | 931 | 3635 | 139 | 3.0 | 13.0 | Fig. 8F–I |
Thin CTB 647 | Palelat CTB 555 | 308-m | 853 | 61 | 2586 | 1194 | 173 | 4.4 | 12.7 | Supplemental Fig. 3e |
Thin DY | Palelat CTB 555 | 308-m | 120 | 93.6 | 2199 | 1006 | 96 | 2.9 | 8.7 | Supplemental Fig. 3e |
Thick CTB 488 | Thick CTBg | 308-m | −127 | 76.3 | 327 | 107 | 97 | 18.3 | 47.5 | Supplemental Fig. 3e |
Palemed DY | Palemed CTB 555 | 293 | −455 | 94.6 | 4777 | 255 | 1255 | 20.0 | 83.1 | Supplemental Figs. 4, 5e |
Thin DY | Thin CTB 647 | 308-m | 318 | 57.4 | 972 | 1448 | 235 | 8.9 | 19.5 | Supplemental Fig. 3e |
Thick CTB 488 | Thin DY | 308-m | 588 | 55.9 | 666 | 736 | 11 | 0.8 | 1.6 | Supplemental Fig. 3e |
↵aDistance between the lateral edge of medial-most injection site and medial edge of lateral-most injection site, measured across CO stripes (negative values indicate that the two injections partially overlap in the mediolateral plane, albeit not in the anteroposterior plane).
↵bArea of overlap of the two labels/smallest area of single labeled field × 100.
↵cCalculated as follows: no. double-labeled cells*100/(no. tracer 1-labeled cells + no. tracer 2-labeled cells + no. double-labeled cells).
↵dCalculated as follows: no. double-labeled cells*100/(smallest no. of cells labeled with a single tracer + no. double-labeled cells).
↵eAvailable at www.jneurosci.org as supplemental material.