Table 1.

ACh EC₅₀, ANOVA, and n_H for the Cys mutants

Construct	EC₅₀ (μm)	ANOVA^a	n_H	n
αβγδ
wild type	2.96 ± 0.54		1.30 ± 0.11	4
αY277C	8.42 ± 0.72	**	1.15 ± 0.04	3
αK276C	4.42 ± 0.46	ns	1.31 ± 0.02	3
αG275C	0.64 ± 0.03	***	1.18 ± 0.06	3
αI274C	34.8 ± 4.5	***	1.34 ± 0.02	3
αL273C	1.45 ± 0.15	ns	1.36 ± 0.03	3
αP272C	1.34 ± 0.07	ns	1.42 ± 0.06	3
αV271C	2.74 ± 0.17	ns	1.47 ± 0.21	3
αA270C	2.37 ± 0.60	ns	1.08 ± 0.14	4
αS269C	0.18 ± 0.03	***	1.41 ± 0.06	4
αS268C	0.79 ± 0.13	***	1.45 ± 0.08	5
αT267C	28.0 ± 5.2	***	1.37 ± 0.07	5
αS266C	17.0 ± 2.9	***	1.26 ± 0.08	3
αP265C	3.25 ± 0.43	ns	1.11 ± 0.04	3
αI264C	9.0 ± 1.6	***	1.38 ± 0.05	8
αL263C	0.40 ± 0.06	***	1.41 ± 0.05	4
αE262C	2.52 ± 0.40	ns	1.27 ± 0.06	3

The two mutants that varied most from wild type and for which the dose–response curves are shown in Figure 2 are in bold.
↵^aOne-way ANOVA of log(EC₅₀) with Dunnett's posttest; ns, p > 0.05, not significant; **0.001 < p < 0.01, very significant; ***p < 0.0001, extremely significant.