Compositions of pipette solutions for dose–response experiments
| Free Ca2+ (m) | NMDG-Cl (mm) | CaCl2 (mm) | EGTA (mm) | HEDTA (mm) | NTA (mm) |
|---|---|---|---|---|---|
| 2 × 10−8 | 153 | 1 | 11 | — | — |
| 1 × 10−7 | 147.8 | 3.6 | 10 | — | — |
| 2.5 × 10−7 | 143.4 | 5.8 | 10 | — | — |
| 5 × 10−7 | 140.2 | 7.4 | 10 | — | — |
| 1 × 10−6 | 152.4 | 1.3 | — | 10 | — |
| 1 × 10−5 | 143 | 6 | — | 10 | — |
| 2.5 × 10−5 | 139.2 | 7.9 | — | 10 | — |
| 1 × 10−4 | 151.4 | 1.8 | — | — | 5 |
| 1 × 10−3 | 153 | 1 | — | — | — |
All solutions contained 10 mm HEPES. The pH was adjusted to 7.2 with NMDGOH, and osmotic pressure was 360 mosmol/L. Free Ca2+ concentrations were calculated with WebmaxC Standard (http://www.stanford.edu/∼cpatton/webmaxc/webmaxcS.htm).