Morphological parameters and opsin levels in retinae of adult and aged B6D2F1/J mice
| Adult mice (4-month-old) | Aged mice (2.5-year-old) | p value | |
|---|---|---|---|
| ONL thickness, μm | 29.4 ± 0.5 (n = 196; 3 mice) | 25.1 ± 0.4 (n = 196; 3 mice) | <0.001 |
| Rod nuclei, per 50 μm | 98.5 ± 1.7 (n = 196; 3 mice) | 78.4 ± 1.4 (n = 196; 3 mice) | <0.001 |
| ROS length, μm | 21.3 ± 0.2 (n = 196; 3 mice) | 19.3 ± 0.3 (n = 196; 3 mice) | <0.001 |
| ROS diameter, μm | 1.53 ± 0.01 (n = 2335; 3 mice) | 1.46 ± 0.01 (n = 2134; 3 mice) | <0.001 |
| Opsin level, pmol/retina | 472.5 ± 19.3 (n = 4) | 230.7 ± 38.2 (n = 4) | <0.002 |
Data from light microscopy were averaged across all 14 analyzed retina locations (7 dorsal and 7 ventral). ROS diameter was measured by TEM in ultrathin cross-sections, including 1000 μm of retinal length, starting approximately 500 μm lateral to the optic nerve. The total number of measured profiles is given. Values are means ± SEM.