Media | Source | |
---|---|---|
ES cell culture media | ||
GMEM | 500 ml | Sigma-Aldrich; G5154 |
FBS | 10% | HyClone; SH30071.03 |
Glutamine | 2 mm | Invitrogen; 25030-081 |
NEAA | 0.1 mm | Invitrogen; 11140-050 |
Sodium pyruvate | 1 mm | Invitrogen; 11360-070 |
2-Mercaptoethanol | 55 μm | Invitrogen; 21985-023 |
LIF | 2000 U/ml | Conditioned media |
Pen/Strep | 100 U/ml | Invitrogen; 15140-122 |
MS5 culture media | ||
αMEM | 500 ml | Invitrogen; 12571-063 |
FBS | 10% | Invitrogen; 16140-071 |
Pen/Strep | 100 U/ml | Invitrogen; 15140-122 |
MS5 and ES differentiation media | ||
GMEM | 500 ml | Invitrogen; 11710-035 |
KSR | 10% | Invitrogen; 10828-028 |
NEAA | 0.1 mm | Invitrogen; 11140-050 |
Sodium pyruvate | 1 mm | Invitrogen; 11360-070 |
2-Mercaptoethanol | 55 μm | Invitrogen; 21985-023 |
Pen/Strep | 100 U/ml | Invitrogen; 15140-122 |
Abbreviations: GMEM, Glasgow minimal essential medium; NEAA, nonessential amino acids; KSR, knock-out serum replacement; αMEM, α minimum essential medium.