Zif268 expression after (15 CS presentations) extinction retrieval in mice fed control or ZnR diets 3 weeks prior to conditioning
| Region (distance from bregma) | Unconditioned | Conditioned | Strain–diet–protocol interaction | ||||||
|---|---|---|---|---|---|---|---|---|---|
| S1 | B6 | S1 | B6 | ||||||
| Ctl | ZnR | Ctl | ZnR | Ctl | ZnR | Ctl | ZnR | ||
| Cortical regions | |||||||||
| M1, 1.78 mm | 12.4 ± 0.9 | 13.3 ± 0.8 | 11.6 ± 0.7 | 13.1 ± 0.6 | 14.1 ± 0.5 | 13.8 ± 0.7 | 13.1 ± 0.7 | 13.0 ± 1.0 | F(1, 40) = 0.02, p = 0.88 |
| M2, 1.78 mm§ | 14.8 ± 0.7 | 14.1 ± 0.8 | 14.3 ± 0.9 | 14.6 ± 0.9 | 22.8 ± 0.6 | 22.5 ± 0.9 | 22.4 ± 0.6 | 22.5 ± 0.5 | F(1, 40) = 0.07, p = 0.79 |
| Cg1, 1.78 mm§ | 16.5 ± 0.3 | 17.6 ± 0.6 | 14.1 ± 1.0 | 15.9 ± 0.3 | 22.0 ± 0.8 | 22.7 ± 0.7 | 23.4 ± 0.9 | 23.4 ± 0.7 | F(1, 40) = 0.31, p = 0.58 |
| Cg1, 1.54 mm§ | 11.6 ± 0.4 | 12.1 ± 0.6 | 13.3 ± 0.6 | 12.1 ± 0.1 | 13.8 ± 0.6 | 14.3 ± 0.8 | 13.8 ± 0.4 | 13.2 ± 0.3 | F(1, 40) = 0.26, p = 0.62 |
| Cg2, 1.10 mm§ | 13.9 ± 0.4 | 13.9 ± 0.2 | 13.9 ± 0.3 | 14.1 ± 0.3 | 20.7 ± 0.8 | 20.0 ± 0.9 | 19.6 ± 0.6 | 19.9 ± 0.6 | F(1, 40) = 0.09, p = 0.77 |
| PrL, 1.98 mm§ | 10.3 ± 0.1 | 10.5 ± 0.3 | 10.7 ± 0.6 | 9.6 ± 0.7 | 15.8 ± 1.0 | 15.5 ± 0.8 | 15.4 ± 0.9 | 15.3 ± 1.0 | F(1, 40) = 0.29, p = 0.60 |
| PrL, 1.78 mm§ | 12.9 ± 0.6 | 12.8 ± 0.7 | 10.8 ± 0.6 | 12.6 ± 0.5 | 21.2 ± 1.2 | 22.0 ± 0.6 | 21.7 ± 0.4 | 21.3 ± 0.8 | F(1, 40) = 1.66, p = 0.21 |
| PrL, 1.54 mm§ | 14.3 ± 0.2 | 13.5 ± 0.7 | 12.0 ± 1.1 | 11.7 ± 0.4 | 23.1 ± 1.1 | 22.7 ± 0.9 | 22.8 ± 0.7 | 22.2 ± 0.8 | F(1, 40) = 0.02, p = 0.90 |
| IL, 1.98 mm§ | 7.8 ± 0.3 | 8.0 ± 0.3‡‡ | 8.0 ± 0.3‡‡ | 7.6 ± 0.4‡‡ | 9.4 ± 0.5 | 14.8 ± 0.7## | 15.3 ± 0.6** | 14.3 ± 0.7†† | F(1, 40) = 9.61, p < 0.01 |
| IL, 1.78 mm§ | 6.8 ± 0.5‡‡ | 6.3 ± 0.6‡‡ | 7.3 ± 0.2‡‡ | 7.2 ± 0.7‡‡ | 9.7 ± 0.6 | 17.7 ± 0.4## | 16.4 ± 0.9** | 16.3 ± 0.4†† | F(1, 40) = 18.11, p < 0.01 |
| IL, 1.54 mm§ | 9.3 ± 0.9‡ | 8.4 ± 0.4‡‡ | 8.3 ± 0.7‡‡ | 8.0 ± 0.6‡‡ | 12.9 ± 0.9 | 25.1 ± 1.0## | 21.1 ± 0.9** | 22.2 ± 1.4†† | F(1, 40) = 16.99, p < 0.01 |
| AI, 1.98 mm§ | 9.6 ± 0.6 | 9.9 ± 0.4 | 9.4 ± 0.5 | 9.0 ± 0.5 | 15.3 ± 0.9 | 14.4 ± 0.6 | 14.6 ± 0.7 | 14.9 ± 0.8 | F(1, 40) = 0.54, p = 0.47 |
| AID, 1.78 mm§ | 6.5 ± 0.3 | 6.8 ± 0.5 | 6.4 ± 0.4 | 6.9 ± 0.4 | 11.6 ± 0.9 | 11.9 ± 1.1 | 11.4 ± 1.0 | 11.7 ± 0.7 | F(1, 40) = 0.13, p = 0.72 |
| AID, 1.54 mm§ | 5.8 ± 0.7 | 5.9 ± 0.4 | 5.2 ± 0.3 | 5.8 ± 0.5 | 12.8 ± 0.8 | 12.5 ± 0.8 | 12.7 ± 0.7 | 13.3 ± 0.6 | F(1, 40) =< 0.01, p = 0.96 |
| AIV, 1.78 mm§ | 11.8 ± 0.4 | 11.4 ± 0.9 | 11.4 ± 0.9 | 11.7 ± 0.5 | 16.5 ± 1.0 | 14.8 ± 0.7 | 15.2 ± 0.8 | 14.7 ± 0.6 | F(1, 40) = 0.19, p = 0.73 |
| AIV, 1.54 mm§ | 10.1 ± 0.5 | 9.0 ± 0.4 | 9.9 ± 0.3 | 9.8 ± 0.5 | 14.4 ± 1.0 | 15.0 ± 1.0 | 14.6 ± 0.6 | 14.4 ± 0.8 | F(1, 40) = 0.21, p = 0.65 |
| PRh, −1.58 mm§ | 7.9 ± 0.3 | 6.5 ± 0.5 | 7.3 ± 0.7 | 7.3 ± 0.5 | 18.7 ± 1.7 | 18.1 ± 1.1 | 18.2 ± 1.0 | 18.4 ± 0.9 | F(1, 40) = 0.03, p = 0.87 |
| Ect, −1.58 mm§ | 16.8 ± 0.6 | 16.1 ± 0.8 | 16.1 ± 0.5 | 15.8 ± 0.8 | 18.4 ± 1.1 | 18.1 ± 0.4 | 18.3 ± 0.3 | 17.4 ± 1.0 | F(1, 40) = 0.20, p = 0.66 |
| Amygdala nuclei (all −1.58 mm) | |||||||||
| Lad§ | 16.2 ± 0.5 | 16.4 ± 1.6‡‡ | 15.6 ± 0.5‡‡ | 15.9 ± 0.8‡‡ | 13.6 ± 0.9 | 22.4 ± 1.3## | 20.6 ± 1.0** | 20.5 ± 0.7†† | F(1, 40) = 10.23, p < 0.01 |
| Lav§ | 6.8 ± 0.4‡ | 7.5 ± 0.4‡‡ | 7.1 ± 0.3‡‡ | 6.9 ± 0.3‡‡ | 9.6 ± 0.8 | 16.4 ± 1.0## | 15.9 ± 0.6** | 16.1 ± 1.0†† | F(1, 40) = 5.78, p = 0.02 |
| BA§ | 3.5 ± 0.1‡‡ | 3.3 ± 0.3‡‡ | 3.7 ± 0.4‡‡ | 4.2 ± 0.3‡‡ | 7.6 ± 1.3 | 13.5 ± 0.7## | 13.3 ± 0.3** | 12.8 ± 0.7†† | F(1, 40) = 13.05, p < 0.01 |
| CeMa§ | 33.1 ± 0.8‡‡ | 35.6 ± 1.5‡‡ | 34.1 ± 2.2‡‡ | 34.5 ± 1.3‡‡ | 74.3 ± 1.3 | 45.6 ± 1.4## | 49.8 ± 4.0** | 49.1 ± 2.5†† | F(1, 40) = 17.48, p < 0.01 |
| CeLa§ | 49.5 ± 2.5‡‡ | 48.3 ± 3.3‡‡ | 46.5 ± 3.0‡‡ | 48.8 ± 3.2‡‡ | 71.5 ± 3.5 | 90.6 ± 1.9## | 89.0 ± 3.1** | 94.6 ± 4.0†† | F(1, 40) = 3.642, p = 0.04 |
| CeCa§ | 40.8 ± 2.3 | 40.3 ± 2.8 | 41.8 ± 2.1 | 42.0 ± 2.3 | 46.7 ± 0.8 | 45.6 ± 1.6 | 45.6 ± 1.6 | 47.0 ± 2.0 | F(1, 40) = 0.08, p = 0.78 |
| Impa§ | 38.1 ± 1.8‡‡ | 40.0 ± 2.1‡‡ | 40.8 ± 1.4‡‡ | 38.5 ± 2.2‡‡ | 75.9 ± 3.9 | 40.8 ± 2.8## | 44.9 ± 2.0** | 42.3 ± 2.3†† | F(1, 40) = 18.19, p < 0.01 |
| Ilpa§ | 29.0 ± 4.4 | 28.8 ± 3.8 | 29.3 ± 1.8 | 30.0 ± 2.3 | 41.3 ± 3.9 | 40.9 ± 5.3 | 39.9 ± 1.7 | 37.9 ± 3.5 | F(1, 40) = 0.05, p = 0.83 |
| Ina§ | 51.6 ± 6.2 | 52.5 ± 5.7‡‡ | 50.8 ± 4.0‡‡ | 51.0 ± 2.8‡‡ | 52.9 ± 7.6 | 90.8 ± 2.4## | 91.1 ± 3.6** | 93.1 ± 2.1†† | F(1, 40) = 5.90, p = 0.02 |
| MePD§ | 6.9 ± 0.6 | 7.3 ± 0.7 | 6.8 ± 0.4 | 6.6 ± 0.3 | 9.8 ± 0.6 | 10.6 ± 0.7 | 10.8 ± 0.8 | 10.3 ± 0.8 | F(1, 40) = 0.03, p = 0.87 |
| MePV§ | 11.2 ± 0.5 | 10.0 ± 0.5 | 10.8 ± 0.5 | 10.6 ± 0.6 | 13.8 ± 1.2 | 14.4 ± 0.7 | 13.4 ± 0.8 | 13.6 ± 0.6 | F(1, 40) = 0.29, p = 0.60 |
| ACo§ | 17.9 ± 2.0 | 17.9 ± 1.8 | 17.1 ± 1.1 | 17.7 ± 0.7 | 19.3 ± 0.9 | 19.4 ± 0.6 | 18.6 ± 1.2 | 18.8 ± 1.1 | F(1, 40) = 0.01, p = 0.93 |
| PLCo§ | 10.0 ± 0.7 | 9.8 ± 0.9 | 9.6 ± 0.2 | 9.9 ± 0.5 | 16.8 ± 0.6 | 17.9 ± 1.0 | 17.8 ± 0.9 | 18.5 ± 0.6 | F(1, 40) = 0.10, p = 0.75 |
↵aWhole (sub)nucleus counted.
↵**p < 0.01, S1 control diet versus B6 control diet;
↵##p < 0.01, S1 control diet versus ZnR S1;
↵††p < 0.01, S1 control diet versus ZnR B6;
↵‡p < 0.05,
↵‡‡p < 0.01, within-strain and -diet conditioned versus unconditioned;
↵§p < 0.05, conditioned versus non-conditioned (significant effect of protocol).
Bold indicates significant strain–diet ANOVA interactions. Data are mean ± SEM cell numbers. n = 8 per group for conditioned and n = 4 per group for unconditioned mice. ACo, Anterior cortical; AI, insular cortex; AIV, insular cortex, ventral; CeC, central, capsular; Cg1, cingulate area 1; Cg2, cingulate area 2; Ctl, control diet; Ect, ectorhinal cortex; M1, primary motor; M2, secondary motor; MePD, medial, posterodorsal; MePV, medial, posteroventral; PLCo, posterolateral cortical; PRh, perirhinal cortex.