Primer sequences used in the quantitative PCR assays
| Primer | Sequence |
|---|---|
| TNF-α | FW: TCTGCTTGGTGGTTTGCTACGAC |
| RV: TGAGTACCTGGACTTGCAGAACG | |
| IL-1β | FW: GGGTTCCATGGTGAAGTCAAC |
| RV: AAGACCTTCTACCTGAGGAACAACC | |
| IκB-α | FW: TGTAGATGCCTCTCCAAGGATGG |
| RV: CTCATGGACTGATTATGGACAGGAC | |
| IL1ra | FW: GCCCAAGAACACATTCCGAAAGTC |
| RV: AAATGGGCTCCCTCTCATCAGTTC | |
| MKP-1 | FW: ACTTCCCTGTCTCCATCCCT |
| RV: ACGTGAAACTCCACACCTCC | |
| HPRT | FW: CTCATGGACTGATTATGGACAGGAC |
| RV: GCAGGTCAGCAAAGAACTTATAGCC |
FW, Forward; RV, reverse.