Table 1.

Primers used for qPCR assay

Gene name	Gene symbol	GenBank	Sequence	Amplicon (bp)
β-Actin	β-act	FJ915059	F, 5′CATCCATCGTTCACAGGAAGTG 3′	83
			R, 5′TGGTCGTTCGTTTGAATCTCAT 3′
18S ribosomal	18S	FJ915075	F, 5′ACGAGACTCCGGCATGCTA 3′	84
			R, 5′TGGCTGAACGCCACTTGTC 3′
Elongation factor 1 α subunit	EF1a	FJ915061	F, 5′CTGCCAGTGTTGCCTTCGT 3′	105
			R, 5′CCTTGCGCTCAATCTTCCA 3′
β-2 microglobulin	b2m	FJ915076	F, 5′TGCAGCGTTCGACACATGA 3′	102
			R, 5′CACGAATCCATCGCTCCAT 3′
Brain-derived neurotrophic factor	Bdnf	FJ915060	F, 5′TCGAAGGACGTTGACCTGTATG 3′	112
			R, 5′TGGCGGCATCCAGGTAGT 3′
Peptide YYa	Pyy	FJ915073	F, 5′TCCTCATCTGCGTGCTTCTGT 3′	112
			R, 5′GCGGTGTAATATTTGGCGAGTT 3′
Calbindin 2	calb2	FJ915062	F, 5′ACGACAAGGATGGCAATGGT 3′	130
			R, 5′AGCGCCATGATGCTCTTCTT 3′
GABA_A receptor, α1	gabra1	FJ915065	F, 5′TCAGGCAGAGCTGGAAGGAT 3′	117
			R, 5′TGCCGTTGTGGAAGAACGT 3′
Glutamate decarboxylase 1	gad1	FJ915066	F, 5′AACTCAGGCGATTGTTGCAT 3′	109
			R, 5′TGAGGACATTTCCAGCCTTC 3′
Glycine receptor αZ4 subunit	glra4b	FJ915068	F, 5′CTCTGAAGCTGCTGCCATGTT 3′	91
			R, 5′GATCCGTTTCGCTTGTTCCA 3′
Parvalbumin isoform 2a	pvalb5	FJ915072	F, 5′GAATCATGGCACTTGCAGGAA 3′	81
			R, 5′TGAAAGATCCTTGCGCTTGAC 3′

Real-time qPCR primer sequences, GenBank accession number, and amplicon size for the seven genes of interest and the four endogenous genes used in SYBR Green assay. F, Forward; R, reverse.