Effect of test substances on the time constant (τ) and rate constant (k) of sealing
| Test substance | <50 μm τ (min) | <50 μm k (min−1) | R2 < 50 μm | > 50 μm τ (min) | >50 μm k (min−1) | R2 > 50 μm |
|---|---|---|---|---|---|---|
| Control | 5.95 | 0.168 | 0.9865 | 2.16 | 0.461 | 0.9970 |
| db-cAMP | 1.98** | 0.503** | 0.9877 | 0.622** | 1.61** | 0.9976 |
| db-cAMP (no Ca2+) # | 11.62*** | 0.086*** | 0.9455 | 3.98** | 0.251** | 0.9877 |
| cBiMPS | 1.24** | 0.805** | 0.9413 | 0.497** | 2.01** | 0.8231 |
| Epac-cAMP | 2.89** | 0.345** | 0.9141 | 0.628** | 1.59** | 0.7933 |
| DMSO | 7.59 | 0.131 | 0.9798 | 2.15 | 0.461 | 0.9856 |
| KT5720 | 20.1*** | 0.049*** | 0.9769 | 9.91*** | 0.10*** | 0.9769 |
| PKI | 25.9*** | 0.038*** | 0.9972 | 10.7*** | 0.093*** | 0.9676 |
| Epac-cAMP plus PKI | 7.37 | 0.138 | 0.9795 | 3.35 | 0.298 | 0.9404 |
| nPKCη PSF | 6.59** | 0.152*** | 0.9618 |
Statistical comparisons of rate constants (k, min−1) or exponential time constants (τ, min) for sealing of B104 cells transected nearer to (<50 μm) or farther from (>50 μm) the soma. Cells transected nearer to the soma and bathed in a given test substance always sealed at a slower rate compared to cells transected farther from the soma and bathed in the same test substance. R2 < 50 μm or R2 > 50 μm represents the goodness of fit values obtained from a single exponential model to fit the sealing frequency at all PC times for control sealing or for sealing in a given test substance. Asterisks indicate the level of significant difference between sealing time constants or rate constants for a given test substance compared to control sealing. Significance levels for this and all other tables and figures are indicated beside each value as follows: no asterisk = p > 0.05 and p < 0.95; single asterisk (*) = p < 0.05; double asterisk (**) = p < 0.01; and triple asterisk (***) = p < 0.001.
↵#Initial increase in sealing frequency.