Region | POMC cells | DoublePOMC/Fos | Percentage of doublePOMC/Fos | β-endorphin cells | Double β-endorphin/IR | Percentage of doubleβ-endorphin/IR |
---|---|---|---|---|---|---|
RCA | 17.0 ± 3.2 | 1.3 ± 0.3a | 9.4 ± 4.4a | 15.7 ± 2.0 | 4.3 ± 0.8 | 27.9 ± 3.8 |
13.4 ± 1.8 | 4.4 ± 0.7b | 33.6 ± 3.0b | ||||
Arc1 | 37.3 ± 4.3 | 0.7 ± 0.6a | 1.7 ± 0.9a | 43.0 ± 3.8 | 11.3 ± 0.9 | 26.4 ± 0.6 |
41.0 ± 6.8 | 10.4 ± 2.0b | 25.0 ± 4.5b | ||||
Arc2 | 16.6 ± 2.0 | 1.0 ± 0.6a | 5.3 ± 2.9a | 17.0 ± 1.5 | 6.3 ± 1.5 | 36.4 ± 4.4 |
22.0 ± 5.5 | 4.2 ± 0.8b | 19.3 ± 2.6b | ||||
Arc3 | 7.3 ± 1.5 | 0.0 ± 0.0a | 0.0 ± 0.0a | 5.0 ± 3.0 | 0.6 ± 0.6 | 6.1 ± 4.7 |
6.8 ± 3.5 | 0.2 ± 0.2b | 3.3 ± 3.3b |
Values represent estimates of mean counts of cells ± SEM. The mice received an injection of either leptin (n = 5) or PFS (n = 3) intraperitoneally. We used adjacent sections of the same mice for POMC or insulinR in situ hybridization (35S riboprobes). IR, insulinR; Arc1, arcuate nucleus of the hypothalamus, rostral level; Arc2, arcuate nucleus of the hypothalamus, intermediate level; Arc3, arcuate nucleus of the hypothalamus, caudal level; RCA, retrochiasmatic area.
↵aSaline.
↵bLeptin.