Summary of qPCR data from the repeated cocaine experiment
| Gene symbol | Description | Gene ID | Quantitative PCR |
|---|---|---|---|
| FosB | fra-2; FBJ osteosarcoma oncogene B; Immediate early gene | 308411 | F(2,5) = 62.652, p < 0.001 |
| β-gal+ vs β-gal−: +6.3-fold; n = 2, 3; p < 0.001 | |||
| β-gal+ vs NeuN+: +10.0-fold; n = 2, 3; p < 0.001 | |||
| β-gal− vs NeuN+: p = 0.478 | |||
| Arc | Activity-regulated cytoskeleton-associated protein | 54323 | F(2,6) = 10.374, p < 0.05 |
| β-gal+ vs β-gal−: +3.5-fold; n = 3, 3; p < 0.01 | |||
| β-gal+ vs NeuN+: +3.0-fold; n = 3, 3; p < 0.01 | |||
| β-gal− vs NeuN+: p = 0.807 | |||
| Nr4a3 | Nuclear receptor subfamily 4, group A, member 3; transcription factor | 58853 | F(2,6) = 43.423, p < 0.001 |
| β-gal+ vs β-gal−: +6.5-fold; n = 3, 3; p < 0.001 | |||
| β-gal+ vs NeuN+: +6.5-fold; n = 3, 3; p < 0.001 | |||
| β-gal− vs NeuN+: p = 1.000 | |||
| Pdyn | Prodynorphin; peptide found in D1-expressing striatal neurons | 29190 | F(2,6) = 20.757, p < 0.005 |
| β-gal+ vs β-gal−: +3.8-fold; n = 3, 3; p < 0.005 | |||
| β-gal+ vs NeuN+: +3.2-fold; n = 3, 3; p < 0.005 | |||
| β-gal− vs NeuN+: p = 0.716 | |||
| Drd2 | Dopamine receptor D2 | 24318 | F(2,6) = 14.389, p < 0.01 |
| β-gal+ vs β-gal−: −2.9-fold; n = 3, 3; p < 0.005 | |||
| β-gal+ vs NeuN+: −2.8-fold; n = 3, 3; p < 0.005 | |||
| β-gal− vs NeuN+: p = 0.874 | |||
| Adora2a | Adenosine A2a receptor; found in D2-expressing striatal neurons | 25369 | F(2,6) = 3.963, p = 0.080 |
| β-gal+ vs β-gal−: −2.9-fold; n = 3, 3; p = 0.190 | |||
| β-gal+ vs NeuN+: −4.6-fold; n = 3, 3; p < 0.05 | |||
| β-gal− vs NeuN+: p = 0.230 | |||
| Kcnc1 | Kv4; Kv3.1; Potassium voltage-gated channel, Shaw-related subfamily, member 1 | 25327 | F(2,6) = 2.704, p = 0.145 |
| β-gal+ vs β-gal−: p = 0.10 | |||
| β-gal+ vs NeuN+: p = 0.09 | |||
| β-gal− vs NeuN+: p = 0.941 | |||
| Map2k6 | Mkk6; Mitogen-activated protein kinase kinase 6; activator of p38 MAPK | 114495 | F(2,6) = 38.591, p < 0.001 |
| β-gal+ vs β-gal−: −8.5-fold; n = 3, 3; p < 0.001 | |||
| β-gal+ vs NeuN+: −7.3-fold; n = 3, 3; p < 0.001 | |||
| β-gal− vs NeuN+: p = 0.239 | |||
| Mkp1 | Mkp1; Dual specificity phosphatase 1; dephosphorylates and inactivates p38 MAPK | 114856 | F(2,6) = 7.600, p < 0.05 |
| β-gal+ vs β-gal−: +1.3-fold; n = 3, 3; p < 0.05 | |||
| β-gal+ vs NeuN+: +1.6-fold; n = 3, 3; p < 0.01 | |||
| β-gal− vs NeuN+: p = 0.219 |
One-way ANOVA (F and p values are shown in Quantitative PCR column) was used to compare gene expression in three groups of cells: β-gal-positive neurons and β-gal-negative neurons from the same sensitized rats that received an injection of cocaine 90 min before obtaining tissue, and all neurons (NeuN-positive; NeuN+) from sensitized rats that received an injection of saline 90 min before obtaining tissue. Fold-level changes are relative to control levels in NeuN + cells. Positive fold-level changes indicate higher levels, while negative fold-level changes indicate lower levels, than in NeuN + control samples.