Table 1.

Basic characterization of receptors containing concatemers

Combination	ACh EC₅₀ (μm)			5I A85380 response			10 μm 17β-Estradiol
Combination	Mean	SE	N	Mean	SE	N	Mean	SE	N	p
α4&β2 1:8	6.6	1.4	14	1.44	0.06	11	2.56	0.12	7	0.000
α4&β2 8:1	131	11	25	0.19	0.03	9	2.96	0.30	12	0.000
α/β	105	23	18	0.16	0.01	16	0.85	0.06	5	0.047
β/α	121	16	48	0.44	0.04	62	3.17	0.25	6	0.000
α/β&α4	87	8	33	0.14	0.01	29	1.47	0.07	27	0.000
β/α&α4	140	14	43	0.17	0.01	46	3.77	0.20	11	0.000
α/β&β2	2.9	0.6	12	1.84	0.07	16	1.04	0.06	11	0.560
β/α&β2	4.4	0.8	29	1.59	0.07	34	3.73	0.32	7	0.000

The first column names the constructs injected. The next three columns give data on activation by ACh in terms of the EC₅₀ value provided by a fit of the Hill equation. The three columns headed “5I A85380 response” give data on the response to 1 μm 5I A85380 normalized to the response to 1 mm ACh, for that cell. The columns headed “10 μm 17β-Estradiol” give data for the potentiation by 10 μm 17β-estradiol of a response to a low concentration of ACh; the column headed p gives the probability that the potentiation ratio differs significantly from 1 (no effect), by two-tailed t test.