Kinetic parameters for 5-HT and DA uptake kinetics by hSERT WT and hSERT Y95F mutant
| Construct | 5-HT | DA | ||
|---|---|---|---|---|
| Km (μm) | Vmax (pmol · well−1 · min−1) | Km (μm) | Vmax (pmol · well−1 · min−1) | |
| hSERT WT | 0.76 ± 0.13 | 0.66 ± 0.15 | 78 ± 5.7*** | 2.7 ± 0.65 |
| hSERT Y95F | 0.57 ± 0.12 | 0.56 ± 0.10 | 12 ± 1.0 | 2.4 ± 0.52 |
↵Uptake was performed in transiently transfected COS-7 cells plated in 96-well plates and allowed to proceed for 10 min at room temperature. Values are expressed as mean ± SEM; n = 8. Significance levels are indicated for hSERT WT versus hSERT Y95F by the two-tailed t test (***p < 0.001).