Table 3.

Quantification of structural parameters based on 3-D reconstructions of single contralateral and ipsilateral WT RGC arbors in the dLGN at multiple developmental time points

P4P8/P9P14/P15
Number of reconstructed arborsContralateral1079
Ipsilateral644
Total length (μm)Contralateral498.70 ± 186.30#984.36 ± 154.85*3627.01 ± 842.37
*
Ipsilateral852.93 ± 157.78*5821.28 ± 2433.615364.93 ± 1533.14
Number of branch pointsContralateral2.70 ± 1.50***14.14 ± 2.24*60.89 ± 17.47
**
Ipsilateral7.67 ± 1.31*117.75 ± 58.7976.50 ± 13.92
Average branch length (μm)Contralateral184.91 ± 42.50*66.64 ± 6.2565.47 ± 8.65
Ipsilateral95.85 ± 8.9172.37 ± 16.4974.62 ± 13.92
Arbor area (μm2) (coronal plane)Contralateral4787.21 ± 1542.147507.60 ± 1367.55##15,491.57 ± 3263.96
*
Ipsilateral7606.09 ± 1503.8220,366.27 ± 5039.9124,767.69 ± 4905.53
Arbor density (coronal plane)Contralateral0.15 ± 0.01**0.19 ± 0.01**0.27 ± 0.02
****
Ipsilateral0.20 ± 0.01*0.30 ± 0.050.29 ± 0.01
Arbor area (μm2) (sagittal plane)Contralateral3551.08 ± 1215.815703.62 ± 1331.42*12,673.87 ± 2282.74
#**
Ipsilateral6282.46 ± 1066.68*20,381.20 ± 1066.6827,575.87 ± 8775.13
Arbor density (sagittal plane)Contralateral0.18 ± 0.01***0.25 ± 0.01*0.32 ± 0.02
**##*
Ipsilateral0.22 ± 0.01*0.31 ± 0.040.29 ± 0.02
  • Mean ± SEM of various structural parameters for WT contralateral and ipsilateral retinogeniculate arbors at different ages. Asterisks between columns and rows identify statistically significant difference between ages and genotypes:

  • *p < 0.05;

  • **p < 0.01;

  • ***p < 0.001. For completion, all p values < 0.1 are reported.

  • #p = 0.07;

  • ##p = 0.06.