Table 1.

REST loss-of-function causes multiple changes in gene expression during OPC differentiation

Oligodendrocyte		2A
Gene name	Relative fold difference	Gene name	Relative fold difference
CelsR3	10.48 ± 4.16^**	SCG10	13.73 ± 2.24^**
SCG10	6.85 ± 0.25^**	CelsR3	11.33 ± 3.92^**
Snap25	5.25 ± 0.35^**	Snap25	4.88 ± 1.09^**
NFM	4.89 ± 0.98^**	Nestin	3.65 ± 2.05^*
Myt1	3.61 ± 0.12^**	Myt1L	2.95 ± 0.78^**
βIII tubulin	2.08 ± 0.28^**	βIII tubulin	2.93 ± 0.27^**
Syn1	1.96 ± 0.24^**	ATOH	2.50 ± 0.08^**
Nestin	1.83 ± 0.03^**	DCX	2.42 ± 1.17
Hes5	1.82 ± 0.05^**	NGN3	2.36 ± 0.07^**
Hes1	1.76 ± 0.75	NeuroD1	2.29 ± 1.13
Syt2	1.75 ± 0.95	NF-M	2.03 ± 0.02^**
Calb1	1.71 ± 0.08^**	Myt1	1.98 ± 0.15^**
Myt1L	1.58 ± 0.07^**	Hes1	1.71 ± 0.13^**
Olig2	1.49 ± 0.74	Syn1	1.55 ± 0.29^*
ASCL1	1.40 ± 0.04^**	Nkx2.2	1.48 ± 0.20^**
DCX	1.35 ± 0.06^**	NGN2	1.48 ± 0.02^**
PLP	NC	Calb1	1.47 ± 0.09^**
Sox2	NC	GluR2	1.40 ± 0.07^**
PDGFαR	NC	Olig1	1.39 ± 0.03^**
GluR2	NC	Olig2	1.25 ± 0.11^*
NG2	NC	β-actin	NC
Olig1	NC	Sox2	NC
Sox10	NC	ID4	NC
YY1	NC	YY1	NC
GFAP	NC	ASCL1	NC
CTNNb1	NC	Hes5	NC
Id2	NC	PLP	NC
Nkx2.2	NC	PDGFαR	NC
β-actin	NC	ID2	NC
Id4	NC	CNP	0.74 ± 0.22
MOBP	0.79 ± 0.07^**	NG2	0.68 ± 0.36
MBP	0.74 ± 0.05^**	MOBP	0.66 ± 0.38
MAG	0.72 ± 0.03^**	MBP	0.65 ± 0.05^**
CNP	0.50 ± 0.31^*	MAG	0.58 ± 0.18^**
		GFAP	0.41 ± 0.11^**
		Brca1	0.39 ± 0.02^**

Gene changes during differentiation. OPCs were infected with control- or DnREST-expressing retroviruses and grown in proliferation media for 5 d. Cells were switched to either oligodendrocyte media or 2A differentiation media for 3–4 d. Total RNA was collected, and transcript levels were analyzed by real-time reverse transcription-PCR. Relative fold difference in gene expression in DnREST-infected cells is shown relative to control-infected cells. The SD was taken from four to six total PCR runs from two separate experiments. NC, No change.
↵*p < 0.05,
↵**p < 0.005. When not indicated, the change was not statistically significant.