First antibodies
| Recognized antigen | Host-animal/Ig subtype | Pretreatment incubation conditions | Company/Source |
|---|---|---|---|
| Cdc42 | Mouse IgG3 | 1:100 | Santa Cruz Biotechnology (sc-8401) |
| Cop1 (clone CM1A10) | Mouse IgG1 | 1:100 | Gift from J. E. Rothman, Yale School of Medicine, New Haven CT |
| GFAP | Mouse IgG1 | 1:1000 | Sigma (G3898) |
| GFAP | Rabbit | 1:500 | Dako/Invitrogen (Z0334) |
| GFP | Mouse IgG1 | 1:1000 | Millipore (MAB3580) |
| GFP | Rabbit | 1:1000 | Invitrogen (A6455) |
| GFP | Chick | 1:1000 | Aves Lab (GFP-1020) |
| Iba1 | Rabbit | 1:500 | Wako (019-19741) |
| NeuN | Mouse IgG1 | 1:100 | Millipore (MAB377) |
| γ-Tubulin | Mouse IgG1 | 1:100 | Sigma (T5326) |
| pan-Tubulin | Rat | 1:10 | Gift from the Department of Anatomy and Cell Biology, Ludwig-Maximilians University München |
| RFP | Rabbit | 1:500 | Millipore (AB3216) |
| S100 | Rabbit | 1:100 | Sigma (S2644) |
| S100β | Mouse IgG1 | 1:500 | Sigma (S2532) |